Demonstrating Low Histamine Content in Red Wine Samples

Two Red Wine samples were tested for Histamine using the Cogent Diamond Hydride Column in an LCMS method. The wine shown in Figure B was claimed to have virtually no Histamine but had no analytical evidence to support it. The claim was verified using this simple LCMS Method that produces an excellent Histamine Meak.

The other wine in Figure C had to be diluted 5x, and even still the Histamine Peak was much larger than in Figure B.

The data shows that there are significant quantitative differences in the Histamine content of various wines. Observing these differences is made possible using the Cogent Diamond Hydride Column.

Histamine in Wine Chroamtogram
Histamine chemical structure
Histamine 112.0869 m/z

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid
B: Acetonitrile / 0.1% Formic Acid

Time (minutes) %B
0 80
5 10
7 10
8 80

Post Time: 5 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation:
Fig. A: Histamine Standard
Fig. B: “Low Histamine” Red Wine. Sample was filtered with 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.)
Fig. C: “Regular” Red Wine. In addition to filtering, Sample was diluted 1:5 due to strong Histamine Peak.
t0: 0.9 minutes


No 231 Histamine in Red Wine Analyzed with LCMS pdf 0.4 Mb Download File