Detect Trace Levels of Melamine

The unique chemical properties of Melamine (base functionalities) present challenges in obtaining Retention and adequate Sensitivity, when using standard HPLC Columns. In this Method, Melamine is Analyzed under Aqueous Normal Phase (ANP) conditions with an increased Sensitivity making it possible for detection at Trace Levels.

Melamine m/z 127 (M+H)+

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase: 
I: 60% acetonitrile / 40% DI water / 0.1% formic acid
II: 70% acetonitrile / 30% DI water / 0.1% formic acid
Flow rate: 0.4mL / minute
Detection: ESI – pos – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: 166.7µg / mL of compound in 50% Acetonitrile / DI Water / 0.1% Acetic Acid. Run 1:1000 dilution in 80% Acetonitrile / DI Water / 0.1% Acetic Acid
t0: 1.44 minute

Notes: Several dogs and cats died or were seriously ill after consumption of pet foods which had been manufactured with adulterated wheat glutens. After testing of the wheat glutens it was determined that they contained significant levels of Melamine and / or several related compounds. 


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