Analyzing elemental Sulfur content 

A simple and reproducible method has been developed for analysis of elemental Sulfur. The data below, (an overlay of 5 chromatograms) illustrates how the compound can be adequately retained in HPLC with good precision and peak shapes using this straightforward method. 

Peak: Sulfur

Method Conditions:
Column: Cogent Bidentate C18™, 4μm, 100Å.
Catalog No.: 40018-15P
Dimensions: 4.6 mm x 150 mm
Mobile Phase: (90:10) Acetonitrile / DI water with 0.1% formic acid.
Injection vol.: 3 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 263 nm 
Sample Preparation: 0.5 mg/mL Sulfur (7704-34-9) in DCM

   %RSD: <0.1%
   t0: 1.7 minutes
   K’: 5.2

Notes: This method could be used for determination of elemental Sulphur in soils, using DCM as the extracting solvent.
Note 2: Capacity was determined using the following equation: k = (tR-t0) / tO
            tR = Retention time of an analyte peak
            tO = Retention time of non-retained peak