Elemental Sulfur analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9187)
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April 25, 2023
Tanya

AppNotes Cogent Diamond Hydride / AppNotes Food and Beverages / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Analyzing elemental Sulfur content

A simple and reproducible method has been developed for analysis of elemental Sulfur. The data below, (an overlay of 5 chromatograms) illustrates how the compound can be adequately retained in HPLC with good precision and peak shapes using this straightforward method.

Peak:
Sulfur

Method Conditions:
Column: Cogent Bidentate C18™, 4μm, 100Å.
Catalog No.: 40018-15P
Dimensions: 4.6 mm x 150 mm
Mobile Phase: (90:10) Acetonitrile / DI water with 0.1% formic acid.
Injection vol.: 3 µL
Flow rate: 1.0 mL / minute
Detection: UV @ 263 nm
Sample Preparation: 0.5 mg/mL Sulfur (7704-34-9) in DCM

%RSD: <0.1%
t₀: 1.7 minutes
K’: 5.2

Notes: This method could be used for determination of elemental Sulphur in soils, using DCM as the extracting solvent.
Note 2: Capacity was determined using the following equation: k = (t_R-t₀) / t_O t_R = Retention time of an analyte peak
t_O = Retention time of non-retained peak

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