Tramadol Separation with Enhanced LCMS – AppNote

LC-MS Compatible HPLC Method for Tramadol  Tramadol can be retained by Reversed Phase HPLC  but some reported methods require Phosphate buffer additives in the Mobile Phase, which means incompatibility with LC-MS. In contrast, this Method uses Formic Acid as the Mobile Phase additive. An advantage this method has is that it produces enhanced signal-to-noise compared […]

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Triamterene Analyzed with HPLC – AppNote

Symmetrical Peak Shape without Ion Pair Agents Triamterene has numerous amine functional groups and therefore tails readily in Reversed Phase Methods if ion pair agents are not used in the Mobile Phase. However, these additives not only contribute to a less Robust Method in terms of Reproducibility of analyte Retention, but are also incompatible with […]

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Trimethobenzamide Analyzed with HPLC – AppNote

Separation of Trimethobenzamide with a Simple Isocratic Method  The USP Assay Method of Trimethobenzamide is performed by titration. This alternative HPLC approach is fast, easy, and reliable. It has many advantages over the titration Method, including ease of automation and less operator-dependent results. This simple isocratic Method provides sufficient Retention of the API to allow […]

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Tropicamide and Homatropine Analyzed with HPLC – AppNote

Separation of Anticholinergic Mydriatic Agents In this Method, Tropicamide and Homatropine are separated with a simple Gradient. Homatropine is significantly hydrophobic yet still retains well. Furthermore, the Separation between these two solutes is good, illustrating the Selectivity capabilities of this Method. Data is shown for two lots of Columns in order to demonstrate the robustness […]

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Valaciclovir & Degradants Analyzed with LCMS – AppNote

Separation of Valaciclovir from two Degradants The anti-viral Herpes drug Valaciclovir and its degradation products are analyzed using Aqueous Normal Phase HPLC with MS detection. The Method development was completed using UV detection, where excellent Separation between the Prodrug Valaciclovir and its active converted degradant, Acyclovir, was observed. The Method transfer from UV to MS […]

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Valaciclovir Analyzed with HPLC – AppNote

Separation of Prodrug from a Degradant in Forced Degradation of Valtrex® Tablets In this Application Note, the anti-viral Herpes drug Valaciclovir and its main acid degradant are well separated (Figure A). Valaciclovir is a prodrug and the degradant observed here is believed to be the active form, Acyclovir. Both compounds did not retain very strongly […]

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Valsartan and Hydrochlorothiazide Analyzed with HPLC – AppNote

Improved Gradient Method with Faster Equilibration The USP Assay Method for Valsartan in combination with Hydrochlorothiazide features a 27 minute gradient with a 13 minute re-equilibration for a total run time of 40 minutes. In this method, the run time was a quarter of the USP method, and the Column equilibrates much faster when gradients […]

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Vancomycin Analyzed with HPLC- AppNote

Retention of a Highly Polar Antibiotic As a highly polar compound, Vancomycin is difficult to retain with Reversed Phase Methods. In this Method the compound retains very well as illustrated in the Figure. In addition, the repeatability of the analysis, which is demonstrated by the overlay of five consecutive runs, is excellent. The Mobile Phase […]

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Vancomycin HPLC Method Transferred – AppNote

Benefits of using 2.2 µm Column for Vancomycin Analysis The highly polar antibiotic Vancomycin can be readily analyzed with either the standard 4µm Cogent Diamond Hydride or the Cogent Diamond Hydride 2.o Column. Notably higher efficiency is obtained on the smaller particle size Column. This Method is easy to perform and is LCMS compatible. Three […]

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Vardenafil (Levitra®) Analyzed with HPLC – AppNote

LC-MS Compatible Assay Method Vardenafil in a tablet formulation can be readily assayed with this LC-MS compatible gradient method. The peak tailing factor was close to unity. The compound has several amine groups which can produce tailing with ordinary HPLC Columns that have a number of surface Silanols. The MS-compatible Mobile Phase means that the […]

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Verapamil and Impurity Analyzed with HPLC – AppNote

Alternative to USP Method with Improved Peak Efficiency  The USP impurities Method for Verapamil uses an Acetate Buffer with the ion pair agent 2-Aminoheptane to reduce Peak Tailing of the API and its Impurity. This Application Note uses an LCMS compatible Mobile Phase and produces Symmetrical Peaks for both Compounds. Excellent Resolution was also obtained […]

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Vitamin K Isomers Analyzed with HPLC – AppNote

Phytonadione Separation by Shape Selectivity In this Method, we separate the two E and Z isomers of Phytonadione on the basis of shape selectivity. The below chromatogram is a five injection overlay with a resolution value of 1.5.  Peaks: 1. Impurity 2. Phytonadione (E isomer) 3. Phytonadione (Z isomer) Method Conditions Column: Cogent UDC-Cholesterol™, 4μm, […]

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Warfarin in a Tablet Analyzed with HPLC – AppNote

Excellent Peak Shape with an Easy to Run Method This Method is easy to prepare, use and reproduce. Note the Excellent Peak Shape for this Compound. This Methodology will give a long Column life and many Reproducible Runs. Peak: Warfarin Method Conditions Column: Cogent Bidentate C18™, 4μm, 100Å Catalog No.: 40018-75P Dimensions: 4.6 x 75mm […]

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Warfarin, Hydroxybupropion, and Codeine by LCMS – AppNote

Analysis in Spiked Blood Samples In this application, three pharmaceuticals/metabolites are separated using the Cogent Diol 2.o Column. The blood sample illustrates the suitability of the Column for analysis of more complex matrices. Use of Acetone in the Mobile Phase was found to be appropriate for this LC-MS method. As a less expensive and less […]

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Water Soluble Vitamin Tablet Analysis with HPLC – AppNote

Separation of Water Soluble Vitamins without Ion Pair Agents This AppNote illustrates the Separation of four Hydrophilic, B Vitamins which were extracted from a commercially available tablet formulation. The data shows that these Water soluble B-Vitamins are well Separated from each other and from any Matrix Peaks. The main advantage of this Method over traditional […]

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β-Carotene Capsule Analyzed with HPLC – AppNote

Separation from Matrix Peaks Beta-carotene may be taken as a dietary supplement in capsule form. In this case, a wide variety of Matrix Peaks were observed in the chromatographic data. It is possible that some of these peaks are various isomers of all-trans ß-carotene or other similar carotenes. In any case, resolution was obtained from […]

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