Orthogonal HPLC Method Development – AppNote – MicroSolv Technology Corporation

collapsCat options: Array ( [title] => Article Categories [showPostCount] => 0 [inExclude] => exclude [inExcludeCats] => default-title,uncategorized,featured-articles,180,1651,ce-accessories [showPosts] => 0 [showPages] => 0 [linkToCat] => 1 [olderThan] => 0 [excludeAll] => 0 [catSortOrder] => ASC [catSort] => catName [postSortOrder] => ASC [postSort] => postTitle [expand] => 4 [defaultExpand] => [debug] => 1 [postTitleLength] => 0 [catfeed] => none [taxonomy] => category [post_type] => post [postDateAppend] => after [postDateFormat] => m/d [showPostDate] => 0 [useCookies] => 0 [postsBeforeCats] => 0 [expandCatPost] => 1 [showEmptyCat] => 1 [showTopLevel] => 1 [useAjax] => 0 [customExpand] => [customCollapse] => [style] => kubrick [accordion] => 1 [title_link] => [addMisc] => 0 [addMiscTitle] => [number] => 2 [includeCatArray] => Array ( ) [expandSym] => [collapseSym] => ) postsToExclude: Array ( ) CATEGORY QUERY RESULTS Array ( [0] => WP_Term Object ( [term_id] => 165 [name] => 2.o™ HPLC Columns FAQ [slug] => 127 [term_group] => 0 [term_taxonomy_id] => 165 [taxonomy] => category [description] => [parent] => 153 [count] => 8 [filter] => raw ) [1] => WP_Term Object ( [term_id] => 211 [name] => Accounting - AP/AR Department [slug] => 116 [term_group] => 0 [term_taxonomy_id] => 211 [taxonomy] => category [description] => [parent] => 210 [count] => 22 [filter] => raw ) [2] => WP_Term Object ( [term_id] => 221 [name] => Accounting Dept. Forms [slug] => 124 [term_group] => 0 [term_taxonomy_id] => 221 [taxonomy] => category [description] => [parent] => 211 [count] => 5 [filter] => raw ) [3] => WP_Term Object ( [term_id] => 229 [name] => Accounts Receivable [slug] => 147 [term_group] => 0 [term_taxonomy_id] => 229 [taxonomy] => category [description] => [parent] => 225 [count] => 2 [filter] => raw ) [4] => WP_Term Object ( [term_id] => 154 [name] => Amide FAQ [slug] => 168 [term_group] => 0 [term_taxonomy_id] => 154 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [5] => WP_Term Object ( [term_id] => 106 [name] => AppNotes AgroChem [slug] => 83 [term_group] => 0 [term_taxonomy_id] => 106 [taxonomy] => category [description] => [parent] => 105 [count] => 11 [filter] => raw ) [6] => WP_Term Object ( [term_id] => 113 [name] => AppNotes ANP/HILIC Methods [slug] => 138 [term_group] => 0 [term_taxonomy_id] => 113 [taxonomy] => category [description] => [parent] => 14 [count] => 185 [filter] => raw ) [7] => WP_Term Object ( [term_id] => 107 [name] => AppNotes Chemicals/Energy [slug] => 86 [term_group] => 0 [term_taxonomy_id] => 107 [taxonomy] => category [description] => [parent] => 105 [count] => 24 [filter] => raw ) [8] => WP_Term Object ( [term_id] => 94 [name] => AppNotes Cogent Amide [slug] => 167 [term_group] => 0 [term_taxonomy_id] => 94 [taxonomy] => category [description] => [parent] => 84 [count] => 6 [filter] => raw ) [9] => WP_Term Object ( [term_id] => 97 [name] => AppNotes Cogent Bidentate C18 [slug] => 92 [term_group] => 0 [term_taxonomy_id] => 97 [taxonomy] => category [description] => [parent] => 84 [count] => 92 [filter] => raw ) [10] => WP_Term Object ( [term_id] => 57 [name] => AppNotes Cogent Bidentate C8 [slug] => 97 [term_group] => 0 [term_taxonomy_id] => 57 [taxonomy] => category [description] => [parent] => 84 [count] => 16 [filter] => raw ) [11] => WP_Term Object ( [term_id] => 96 [name] => AppNotes Cogent Bidentate C8 300 [slug] => 100 [term_group] => 0 [term_taxonomy_id] => 96 [taxonomy] => category [description] => [parent] => 84 [count] => 3 [filter] => raw ) [12] => WP_Term Object ( [term_id] => 98 [name] => AppNotes Cogent Diamond Hydride [slug] => 90 [term_group] => 0 [term_taxonomy_id] => 98 [taxonomy] => category [description] => [parent] => 84 [count] => 163 [filter] => raw ) [13] => WP_Term Object ( [term_id] => 99 [name] => AppNotes Cogent Diol [slug] => 146 [term_group] => 0 [term_taxonomy_id] => 99 [taxonomy] => category [description] => [parent] => 84 [count] => 7 [filter] => raw ) [14] => WP_Term Object ( [term_id] => 160 [name] => AppNotes Cogent HPS [slug] => 135 [term_group] => 0 [term_taxonomy_id] => 160 [taxonomy] => category [description] => [parent] => 15 [count] => 4 [filter] => raw ) [15] => WP_Term Object ( [term_id] => 261 [name] => AppNotes Cogent Phenyl Hexyl [slug] => appnotes-cogent-phenyl-hexyl [term_group] => 0 [term_taxonomy_id] => 261 [taxonomy] => category [description] => [parent] => 15 [count] => 1 [filter] => raw ) [16] => WP_Term Object ( [term_id] => 100 [name] => AppNotes Cogent Phenyl Hydride [slug] => 93 [term_group] => 0 [term_taxonomy_id] => 100 [taxonomy] => category [description] => [parent] => 84 [count] => 32 [filter] => raw ) [17] => WP_Term Object ( [term_id] => 262 [name] => AppNotes Cogent RP C8 [slug] => appnotes-cogent-rp-c8 [term_group] => 0 [term_taxonomy_id] => 262 [taxonomy] => category [description] => [parent] => 0 [count] => 3 [filter] => raw ) [18] => WP_Term Object ( [term_id] => 101 [name] => AppNotes Cogent Silica-C [slug] => 98 [term_group] => 0 [term_taxonomy_id] => 101 [taxonomy] => category [description] => [parent] => 84 [count] => 6 [filter] => raw ) [19] => WP_Term Object ( [term_id] => 102 [name] => AppNotes Cogent UDA [slug] => 99 [term_group] => 0 [term_taxonomy_id] => 102 [taxonomy] => category [description] => [parent] => 84 [count] => 7 [filter] => raw ) [20] => WP_Term Object ( [term_id] => 103 [name] => AppNotes Cogent UDC Cholesterol [slug] => 94 [term_group] => 0 [term_taxonomy_id] => 103 [taxonomy] => category [description] => [parent] => 84 [count] => 16 [filter] => raw ) [21] => WP_Term Object ( [term_id] => 108 [name] => AppNotes Food and Beverages [slug] => 84 [term_group] => 0 [term_taxonomy_id] => 108 [taxonomy] => category [description] => [parent] => 105 [count] => 69 [filter] => raw ) [22] => WP_Term Object ( [term_id] => 14 [name] => AppNotes Library [slug] => 63 [term_group] => 0 [term_taxonomy_id] => 14 [taxonomy] => category [description] => [parent] => 4 [count] => 366 [filter] => raw ) [23] => WP_Term Object ( [term_id] => 15 [name] => AppNotes Listed by Column [slug] => 91 [term_group] => 0 [term_taxonomy_id] => 15 [taxonomy] => category [description] => [parent] => 14 [count] => 21 [filter] => raw ) [24] => WP_Term Object ( [term_id] => 104 [name] => AppNotes Listed by Compound [slug] => 96 [term_group] => 0 [term_taxonomy_id] => 104 [taxonomy] => category [description] => [parent] => 14 [count] => 1 [filter] => raw ) [25] => WP_Term Object ( [term_id] => 105 [name] => AppNotes Listed by Discipline [slug] => 95 [term_group] => 0 [term_taxonomy_id] => 105 [taxonomy] => category [description] => [parent] => 14 [count] => 36 [filter] => raw ) [26] => WP_Term Object ( [term_id] => 110 [name] => AppNotes Metabolites [slug] => 85 [term_group] => 0 [term_taxonomy_id] => 110 [taxonomy] => category [description] => [parent] => 105 [count] => 75 [filter] => raw ) [27] => WP_Term Object ( [term_id] => 258 [name] => AppNotes Normal Phase [slug] => appnotes-normal-phase [term_group] => 0 [term_taxonomy_id] => 258 [taxonomy] => category [description] => [parent] => 14 [count] => 3 [filter] => raw ) [28] => WP_Term Object ( [term_id] => 109 [name] => AppNotes Pharmaceuticals [slug] => 82 [term_group] => 0 [term_taxonomy_id] => 109 [taxonomy] => category [description] => [parent] => 105 [count] => 184 [filter] => raw ) [29] => WP_Term Object ( [term_id] => 111 [name] => AppNotes Proteins Peptides [slug] => 87 [term_group] => 0 [term_taxonomy_id] => 111 [taxonomy] => category [description] => [parent] => 105 [count] => 8 [filter] => raw ) [30] => WP_Term Object ( [term_id] => 114 [name] => AppNotes Reversed Phase Methods [slug] => 140 [term_group] => 0 [term_taxonomy_id] => 114 [taxonomy] => category [description] => [parent] => 14 [count] => 153 [filter] => raw ) [31] => WP_Term Object ( [term_id] => 17 [name] => AppNotes RP C18 [slug] => 181 [term_group] => 0 [term_taxonomy_id] => 17 [taxonomy] => category [description] => [parent] => 15 [count] => 11 [filter] => raw ) [32] => WP_Term Object ( [term_id] => 18 [name] => AppNotes RSA Vials [slug] => rsa-glass-vial-applications [term_group] => 0 [term_taxonomy_id] => 18 [taxonomy] => category [description] => [parent] => 14 [count] => 4 [filter] => raw ) [33] => WP_Term Object ( [term_id] => 84 [name] => AppNotes TYPE-C Columns [slug] => cogent-type-c-silica-columns [term_group] => 0 [term_taxonomy_id] => 84 [taxonomy] => category [description] => [parent] => 15 [count] => 0 [filter] => raw ) [34] => WP_Term Object ( [term_id] => 214 [name] => Archived App Notes Internal [slug] => 142 [term_group] => 0 [term_taxonomy_id] => 214 [taxonomy] => category [description] => [parent] => 5 [count] => 26 [filter] => raw ) [35] => WP_Term Object ( [term_id] => 4 [name] => ARTICLES, APPNOTES, FAQ [slug] => 46 [term_group] => 0 [term_taxonomy_id] => 4 [taxonomy] => category [description] => [parent] => 0 [count] => 1210 [filter] => raw ) [36] => WP_Term Object ( [term_id] => 120 [name] => Autosampler Vials FAQ [slug] => 9 [term_group] => 0 [term_taxonomy_id] => 120 [taxonomy] => category [description] => [parent] => 119 [count] => 11 [filter] => raw ) [37] => WP_Term Object ( [term_id] => 250 [name] => Autosampler Vials Information [slug] => autosampler-vial-tips [term_group] => 0 [term_taxonomy_id] => 250 [taxonomy] => category [description] => [parent] => 4 [count] => 116 [filter] => raw ) [38] => WP_Term Object ( [term_id] => 257 [name] => Autosampler Vials Internal [slug] => autosampler-vials [term_group] => 0 [term_taxonomy_id] => 257 [taxonomy] => category [description] => [parent] => 5 [count] => 3 [filter] => raw ) [39] => WP_Term Object ( [term_id] => 156 [name] => Bidentate C18 FAQ [slug] => 22 [term_group] => 0 [term_taxonomy_id] => 156 [taxonomy] => category [description] => [parent] => 153 [count] => 6 [filter] => raw ) [40] => WP_Term Object ( [term_id] => 155 [name] => Bidentate C8 FAQ [slug] => 23 [term_group] => 0 [term_taxonomy_id] => 155 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [41] => WP_Term Object ( [term_id] => 256 [name] => Buffers Discontinued Internal [slug] => buffers-discontinued [term_group] => 0 [term_taxonomy_id] => 256 [taxonomy] => category [description] => [parent] => 7 [count] => 1 [filter] => raw ) [42] => WP_Term Object ( [term_id] => 129 [name] => Buffers for CE FAQ [slug] => 25 [term_group] => 0 [term_taxonomy_id] => 129 [taxonomy] => category [description] => [parent] => 128 [count] => 5 [filter] => raw ) [43] => WP_Term Object ( [term_id] => 208 [name] => Capillary Electrophoresis Information [slug] => 61 [term_group] => 0 [term_taxonomy_id] => 208 [taxonomy] => category [description] => [parent] => 4 [count] => 31 [filter] => raw ) [45] => WP_Term Object ( [term_id] => 128 [name] => CE and CZE FAQ [slug] => 13 [term_group] => 0 [term_taxonomy_id] => 128 [taxonomy] => category [description] => [parent] => 119 [count] => 9 [filter] => raw ) [46] => WP_Term Object ( [term_id] => 255 [name] => CElixir Products [slug] => celixir-products [term_group] => 0 [term_taxonomy_id] => 255 [taxonomy] => category [description] => [parent] => 7 [count] => 112 [filter] => raw ) [48] => WP_Term Object ( [term_id] => 252 [name] => Chrom Syringes FAQ [slug] => chrom-syringes-faq [term_group] => 0 [term_taxonomy_id] => 252 [taxonomy] => category [description] => [parent] => 147 [count] => 5 [filter] => raw ) [49] => WP_Term Object ( [term_id] => 212 [name] => Click To Chat Internal [slug] => 155 [term_group] => 0 [term_taxonomy_id] => 212 [taxonomy] => category [description] => [parent] => 5 [count] => 5 [filter] => raw ) [50] => WP_Term Object ( [term_id] => 207 [name] => Cogent HPS FAQ [slug] => 34 [term_group] => 0 [term_taxonomy_id] => 207 [taxonomy] => category [description] => [parent] => 253 [count] => 6 [filter] => raw ) [51] => WP_Term Object ( [term_id] => 167 [name] => Column Hardware FAQ [slug] => 137 [term_group] => 0 [term_taxonomy_id] => 167 [taxonomy] => category [description] => [parent] => 153 [count] => 16 [filter] => raw ) [52] => WP_Term Object ( [term_id] => 135 [name] => Controlled Flow Capillary FAQ [slug] => 32 [term_group] => 0 [term_taxonomy_id] => 135 [taxonomy] => category [description] => [parent] => 128 [count] => 17 [filter] => raw ) [53] => WP_Term Object ( [term_id] => 249 [name] => Cornerstone Scientific Internal [slug] => cornerstone-scientific [term_group] => 0 [term_taxonomy_id] => 249 [taxonomy] => category [description] => [parent] => 5 [count] => 4 [filter] => raw ) [54] => WP_Term Object ( [term_id] => 127 [name] => Crimp Top Vials & Cap FAQ [slug] => 184 [term_group] => 0 [term_taxonomy_id] => 127 [taxonomy] => category [description] => [parent] => 120 [count] => 8 [filter] => raw ) [55] => WP_Term Object ( [term_id] => 222 [name] => Customer Care Department [slug] => 114 [term_group] => 0 [term_taxonomy_id] => 222 [taxonomy] => category [description] => [parent] => 210 [count] => 2 [filter] => raw ) [57] => WP_Term Object ( [term_id] => 157 [name] => Diamond Hydride FAQ [slug] => cogent-diamond-hydride [term_group] => 0 [term_taxonomy_id] => 157 [taxonomy] => category [description] => [parent] => 153 [count] => 34 [filter] => raw ) [58] => WP_Term Object ( [term_id] => 158 [name] => Diol FAQ [slug] => 145 [term_group] => 0 [term_taxonomy_id] => 158 [taxonomy] => category [description] => [parent] => 153 [count] => 1 [filter] => raw ) [59] => WP_Term Object ( [term_id] => 7 [name] => Discontinued Products Internal [slug] => discontinued-products [term_group] => 0 [term_taxonomy_id] => 7 [taxonomy] => category [description] => [parent] => 5 [count] => 14 [filter] => raw ) [60] => WP_Term Object ( [term_id] => 216 [name] => Employee Cell Phone Internal [slug] => 131 [term_group] => 0 [term_taxonomy_id] => 216 [taxonomy] => category [description] => [parent] => 5 [count] => 1 [filter] => raw ) [61] => WP_Term Object ( [term_id] => 219 [name] => Employee Manual Internal [slug] => 103 [term_group] => 0 [term_taxonomy_id] => 219 [taxonomy] => category [description] => [parent] => 5 [count] => 12 [filter] => raw ) [62] => WP_Term Object ( [term_id] => 139 [name] => Equipment FAQ [slug] => 17 [term_group] => 0 [term_taxonomy_id] => 139 [taxonomy] => category [description] => [parent] => 119 [count] => 9 [filter] => raw ) [63] => WP_Term Object ( [term_id] => 140 [name] => Filter Degasser FAQ [slug] => 20 [term_group] => 0 [term_taxonomy_id] => 140 [taxonomy] => category [description] => [parent] => 147 [count] => 5 [filter] => raw ) [64] => WP_Term Object ( [term_id] => 147 [name] => Filtration FAQ [slug] => 14 [term_group] => 0 [term_taxonomy_id] => 147 [taxonomy] => category [description] => [parent] => 119 [count] => 1 [filter] => raw ) [65] => WP_Term Object ( [term_id] => 220 [name] => Financial Reporting [slug] => 117 [term_group] => 0 [term_taxonomy_id] => 220 [taxonomy] => category [description] => [parent] => 211 [count] => 1 [filter] => raw ) [66] => WP_Term Object ( [term_id] => 152 [name] => Fittings for HPLC FAQ [slug] => 12 [term_group] => 0 [term_taxonomy_id] => 152 [taxonomy] => category [description] => [parent] => 119 [count] => 41 [filter] => raw ) [67] => WP_Term Object ( [term_id] => 119 [name] => Frequently Asked Questions?? [slug] => 8 [term_group] => 0 [term_taxonomy_id] => 119 [taxonomy] => category [description] => [parent] => 4 [count] => 4 [filter] => raw ) [68] => WP_Term Object ( [term_id] => 69 [name] => General Information [slug] => 141 [term_group] => 0 [term_taxonomy_id] => 69 [taxonomy] => category [description] => [parent] => 4 [count] => 13 [filter] => raw ) [69] => WP_Term Object ( [term_id] => 223 [name] => General Policies & Procedure Internal [slug] => 109 [term_group] => 0 [term_taxonomy_id] => 223 [taxonomy] => category [description] => [parent] => 210 [count] => 7 [filter] => raw ) [70] => WP_Term Object ( [term_id] => 159 [name] => Guard Columns FAQ [slug] => 81 [term_group] => 0 [term_taxonomy_id] => 159 [taxonomy] => category [description] => [parent] => 153 [count] => 11 [filter] => raw ) [71] => WP_Term Object ( [term_id] => 123 [name] => Headspace Vial FAQ [slug] => 19 [term_group] => 0 [term_taxonomy_id] => 123 [taxonomy] => category [description] => [parent] => 120 [count] => 17 [filter] => raw ) [72] => WP_Term Object ( [term_id] => 70 [name] => How To... [slug] => 76 [term_group] => 0 [term_taxonomy_id] => 70 [taxonomy] => category [description] => [parent] => 4 [count] => 43 [filter] => raw ) [73] => WP_Term Object ( [term_id] => 76 [name] => HPLC Column Information [slug] => 49 [term_group] => 0 [term_taxonomy_id] => 76 [taxonomy] => category [description] => [parent] => 71 [count] => 25 [filter] => raw ) [74] => WP_Term Object ( [term_id] => 153 [name] => HPLC Columns FAQ [slug] => 11 [term_group] => 0 [term_taxonomy_id] => 153 [taxonomy] => category [description] => [parent] => 119 [count] => 2 [filter] => raw ) [75] => WP_Term Object ( [term_id] => 254 [name] => HPLC Columns Old [slug] => hplc-columns-old [term_group] => 0 [term_taxonomy_id] => 254 [taxonomy] => category [description] => [parent] => 7 [count] => 4 [filter] => raw ) [76] => WP_Term Object ( [term_id] => 71 [name] => HPLC Information and Suggestions [slug] => 51 [term_group] => 0 [term_taxonomy_id] => 71 [taxonomy] => category [description] => [parent] => 4 [count] => 5 [filter] => raw ) [77] => WP_Term Object ( [term_id] => 77 [name] => HPLC Tips and Suggestions [slug] => 18 [term_group] => 0 [term_taxonomy_id] => 77 [taxonomy] => category [description] => [parent] => 71 [count] => 338 [filter] => raw ) [78] => WP_Term Object ( [term_id] => 124 [name] => Inserts FAQ [slug] => 158 [term_group] => 0 [term_taxonomy_id] => 124 [taxonomy] => category [description] => [parent] => 120 [count] => 10 [filter] => raw ) [79] => WP_Term Object ( [term_id] => 5 [name] => INTERNAL ONLY - CONFIDENTIAL [slug] => 102 [term_group] => 0 [term_taxonomy_id] => 5 [taxonomy] => category [description] => [parent] => 0 [count] => 52 [filter] => raw ) [80] => WP_Term Object ( [term_id] => 230 [name] => Inventory Module [slug] => 166 [term_group] => 0 [term_taxonomy_id] => 230 [taxonomy] => category [description] => [parent] => 225 [count] => 2 [filter] => raw ) [81] => WP_Term Object ( [term_id] => 215 [name] => IT Computer Warriors Internal [slug] => 156 [term_group] => 0 [term_taxonomy_id] => 215 [taxonomy] => category [description] => [parent] => 5 [count] => 2 [filter] => raw ) [82] => WP_Term Object ( [term_id] => 145 [name] => Knitted Reactor Coils [slug] => 28 [term_group] => 0 [term_taxonomy_id] => 145 [taxonomy] => category [description] => [parent] => 144 [count] => 15 [filter] => raw ) [83] => WP_Term Object ( [term_id] => 206 [name] => Labelled Vials [slug] => 179 [term_group] => 0 [term_taxonomy_id] => 206 [taxonomy] => category [description] => [parent] => 205 [count] => 1 [filter] => raw ) [84] => WP_Term Object ( [term_id] => 224 [name] => Marketing Department [slug] => 119 [term_group] => 0 [term_taxonomy_id] => 224 [taxonomy] => category [description] => [parent] => 210 [count] => 3 [filter] => raw ) [85] => WP_Term Object ( [term_id] => 122 [name] => MicroSolv Autosampler Vials FAQ [slug] => 43 [term_group] => 0 [term_taxonomy_id] => 122 [taxonomy] => category [description] => [parent] => 120 [count] => 7 [filter] => raw ) [86] => WP_Term Object ( [term_id] => 170 [name] => Mobile Phase FAQ [slug] => 72 [term_group] => 0 [term_taxonomy_id] => 170 [taxonomy] => category [description] => [parent] => 119 [count] => 29 [filter] => raw ) [87] => WP_Term Object ( [term_id] => 78 [name] => Mobile Phase Suggestions [slug] => 58 [term_group] => 0 [term_taxonomy_id] => 78 [taxonomy] => category [description] => [parent] => 71 [count] => 10 [filter] => raw ) [88] => WP_Term Object ( [term_id] => 231 [name] => MTCP [slug] => 112 [term_group] => 0 [term_taxonomy_id] => 231 [taxonomy] => category [description] => [parent] => 210 [count] => 4 [filter] => raw ) [89] => WP_Term Object ( [term_id] => 233 [name] => Online Book of Standards [slug] => 148 [term_group] => 0 [term_taxonomy_id] => 233 [taxonomy] => category [description] => [parent] => 232 [count] => 2 [filter] => raw ) [90] => WP_Term Object ( [term_id] => 2 [name] => ONLINE TOOLS [slug] => 57 [term_group] => 0 [term_taxonomy_id] => 2 [taxonomy] => category [description] => [parent] => 0 [count] => 2 [filter] => raw ) [91] => WP_Term Object ( [term_id] => 232 [name] => Packaging Department [slug] => 111 [term_group] => 0 [term_taxonomy_id] => 232 [taxonomy] => category [description] => [parent] => 210 [count] => 3 [filter] => raw ) [92] => WP_Term Object ( [term_id] => 168 [name] => Partisil & Partisphere FAQ [slug] => 157 [term_group] => 0 [term_taxonomy_id] => 168 [taxonomy] => category [description] => [parent] => 253 [count] => 8 [filter] => raw ) [93] => WP_Term Object ( [term_id] => 144 [name] => Photochemical Reactor [slug] => 27 [term_group] => 0 [term_taxonomy_id] => 144 [taxonomy] => category [description] => [parent] => 139 [count] => 16 [filter] => raw ) [94] => WP_Term Object ( [term_id] => 148 [name] => Pre Column HPLC Filters FAQ [slug] => 37 [term_group] => 0 [term_taxonomy_id] => 148 [taxonomy] => category [description] => [parent] => 147 [count] => 11 [filter] => raw ) [95] => WP_Term Object ( [term_id] => 72 [name] => Prep Chromatography [slug] => 75 [term_group] => 0 [term_taxonomy_id] => 72 [taxonomy] => category [description] => [parent] => 75 [count] => 4 [filter] => raw ) [96] => WP_Term Object ( [term_id] => 244 [name] => Pricing Policies [slug] => pricing-policies [term_group] => 0 [term_taxonomy_id] => 244 [taxonomy] => category [description] => [parent] => 224 [count] => 0 [filter] => raw ) [97] => WP_Term Object ( [term_id] => 210 [name] => Procedures Internal [slug] => 104 [term_group] => 0 [term_taxonomy_id] => 210 [taxonomy] => category [description] => [parent] => 5 [count] => 4 [filter] => raw ) [99] => WP_Term Object ( [term_id] => 234 [name] => Purchasing Department [slug] => 107 [term_group] => 0 [term_taxonomy_id] => 234 [taxonomy] => category [description] => [parent] => 210 [count] => 8 [filter] => raw ) [100] => WP_Term Object ( [term_id] => 171 [name] => Qualification FAQ [slug] => 16 [term_group] => 0 [term_taxonomy_id] => 171 [taxonomy] => category [description] => [parent] => 119 [count] => 59 [filter] => raw ) [101] => WP_Term Object ( [term_id] => 73 [name] => Qualification Information [slug] => 74 [term_group] => 0 [term_taxonomy_id] => 73 [taxonomy] => category [description] => [parent] => 71 [count] => 16 [filter] => raw ) [102] => WP_Term Object ( [term_id] => 242 [name] => Qx2 [slug] => 178 [term_group] => 0 [term_taxonomy_id] => 242 [taxonomy] => category [description] => [parent] => 7 [count] => 1 [filter] => raw ) [103] => WP_Term Object ( [term_id] => 235 [name] => Receiving Department [slug] => 110 [term_group] => 0 [term_taxonomy_id] => 235 [taxonomy] => category [description] => [parent] => 210 [count] => 4 [filter] => raw ) [104] => WP_Term Object ( [term_id] => 253 [name] => Reversed Phase Columns FAQ [slug] => reversed-phase-columns-faq [term_group] => 0 [term_taxonomy_id] => 253 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [105] => WP_Term Object ( [term_id] => 125 [name] => RSA Vials FAQ [slug] => 78 [term_group] => 0 [term_taxonomy_id] => 125 [taxonomy] => category [description] => [parent] => 120 [count] => 19 [filter] => raw ) [106] => WP_Term Object ( [term_id] => 263 [name] => RSA-Pro X FAQ [slug] => rsa-pro-x-faq [term_group] => 0 [term_taxonomy_id] => 263 [taxonomy] => category [description] => [parent] => 120 [count] => 10 [filter] => raw ) [107] => WP_Term Object ( [term_id] => 225 [name] => SAGE 100 [slug] => 121 [term_group] => 0 [term_taxonomy_id] => 225 [taxonomy] => category [description] => [parent] => 210 [count] => 2 [filter] => raw ) [109] => WP_Term Object ( [term_id] => 236 [name] => Sales Department Inbound [slug] => 105 [term_group] => 0 [term_taxonomy_id] => 236 [taxonomy] => category [description] => [parent] => 210 [count] => 23 [filter] => raw ) [110] => WP_Term Object ( [term_id] => 237 [name] => Sales Department Outbound [slug] => 120 [term_group] => 0 [term_taxonomy_id] => 237 [taxonomy] => category [description] => [parent] => 210 [count] => 1 [filter] => raw ) [111] => WP_Term Object ( [term_id] => 226 [name] => Sales Order Module [slug] => 125 [term_group] => 0 [term_taxonomy_id] => 226 [taxonomy] => category [description] => [parent] => 225 [count] => 3 [filter] => raw ) [112] => WP_Term Object ( [term_id] => 3 [name] => SALES POLICIES [slug] => 15 [term_group] => 0 [term_taxonomy_id] => 3 [taxonomy] => category [description] => [parent] => 0 [count] => 10 [filter] => raw ) [113] => WP_Term Object ( [term_id] => 238 [name] => SFDC Salesforce.com [slug] => 106 [term_group] => 0 [term_taxonomy_id] => 238 [taxonomy] => category [description] => [parent] => 210 [count] => 7 [filter] => raw ) [114] => WP_Term Object ( [term_id] => 239 [name] => Shipping Department [slug] => 108 [term_group] => 0 [term_taxonomy_id] => 239 [taxonomy] => category [description] => [parent] => 210 [count] => 17 [filter] => raw ) [115] => WP_Term Object ( [term_id] => 163 [name] => Silica-C FAQ [slug] => 39 [term_group] => 0 [term_taxonomy_id] => 163 [taxonomy] => category [description] => [parent] => 153 [count] => 9 [filter] => raw ) [116] => WP_Term Object ( [term_id] => 150 [name] => Sparging Stones FAQ [slug] => 144 [term_group] => 0 [term_taxonomy_id] => 150 [taxonomy] => category [description] => [parent] => 147 [count] => 16 [filter] => raw ) [117] => WP_Term Object ( [term_id] => 118 [name] => Syringe Filters and Filtration [slug] => 73 [term_group] => 0 [term_taxonomy_id] => 118 [taxonomy] => category [description] => [parent] => 4 [count] => 13 [filter] => raw ) [118] => WP_Term Object ( [term_id] => 149 [name] => Syringe Filters FAQ [slug] => 38 [term_group] => 0 [term_taxonomy_id] => 149 [taxonomy] => category [description] => [parent] => 147 [count] => 30 [filter] => raw ) [119] => WP_Term Object ( [term_id] => 142 [name] => Syringe Pump Handheld [slug] => 45 [term_group] => 0 [term_taxonomy_id] => 142 [taxonomy] => category [description] => [parent] => 7 [count] => 0 [filter] => raw ) [120] => WP_Term Object ( [term_id] => 209 [name] => Technical Support Dept Internal [slug] => 113 [term_group] => 0 [term_taxonomy_id] => 209 [taxonomy] => category [description] => [parent] => 5 [count] => 19 [filter] => raw ) [121] => WP_Term Object ( [term_id] => 228 [name] => Templates Internal [slug] => 122 [term_group] => 0 [term_taxonomy_id] => 228 [taxonomy] => category [description] => [parent] => 5 [count] => 15 [filter] => raw ) [122] => WP_Term Object ( [term_id] => 8 [name] => TG-14 In-line Degasser [slug] => 171 [term_group] => 0 [term_taxonomy_id] => 8 [taxonomy] => category [description] => [parent] => 7 [count] => 24 [filter] => raw ) [123] => WP_Term Object ( [term_id] => 141 [name] => Tips Liquid Handling [slug] => 48 [term_group] => 0 [term_taxonomy_id] => 141 [taxonomy] => category [description] => [parent] => 139 [count] => 5 [filter] => raw ) [124] => WP_Term Object ( [term_id] => 146 [name] => Tools [slug] => 88 [term_group] => 0 [term_taxonomy_id] => 146 [taxonomy] => category [description] => [parent] => 139 [count] => 7 [filter] => raw ) [125] => WP_Term Object ( [term_id] => 217 [name] => Tools & Equipment Internal [slug] => 130 [term_group] => 0 [term_taxonomy_id] => 217 [taxonomy] => category [description] => [parent] => 5 [count] => 3 [filter] => raw ) [126] => WP_Term Object ( [term_id] => 74 [name] => Trouble Shooting Suggestions [slug] => 50 [term_group] => 0 [term_taxonomy_id] => 74 [taxonomy] => category [description] => [parent] => 71 [count] => 69 [filter] => raw ) [127] => WP_Term Object ( [term_id] => 81 [name] => Tubing Information [slug] => 77 [term_group] => 0 [term_taxonomy_id] => 81 [taxonomy] => category [description] => [parent] => 4 [count] => 25 [filter] => raw ) [128] => WP_Term Object ( [term_id] => 75 [name] => TYPE-C Column Information [slug] => 53 [term_group] => 0 [term_taxonomy_id] => 75 [taxonomy] => category [description] => [parent] => 71 [count] => 42 [filter] => raw ) [129] => WP_Term Object ( [term_id] => 164 [name] => TYPE-C in General FAQ [slug] => 40 [term_group] => 0 [term_taxonomy_id] => 164 [taxonomy] => category [description] => [parent] => 153 [count] => 46 [filter] => raw ) [130] => WP_Term Object ( [term_id] => 126 [name] => U-2D Sample MGT FAQ [slug] => 172 [term_group] => 0 [term_taxonomy_id] => 126 [taxonomy] => category [description] => [parent] => 120 [count] => 10 [filter] => raw ) [131] => WP_Term Object ( [term_id] => 162 [name] => UDC-Cholesterol FAQ [slug] => 24 [term_group] => 0 [term_taxonomy_id] => 162 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [133] => WP_Term Object ( [term_id] => 260 [name] => Vial Centrifuge [slug] => vial-centrifuge [term_group] => 0 [term_taxonomy_id] => 260 [taxonomy] => category [description] => [parent] => 5 [count] => 1 [filter] => raw ) [134] => WP_Term Object ( [term_id] => 121 [name] => Vials & Caps General FAQ [slug] => 10 [term_group] => 0 [term_taxonomy_id] => 121 [taxonomy] => category [description] => [parent] => 120 [count] => 64 [filter] => raw ) [135] => WP_Term Object ( [term_id] => 218 [name] => VOIXIS Phone Internal [slug] => 129 [term_group] => 0 [term_taxonomy_id] => 218 [taxonomy] => category [description] => [parent] => 5 [count] => 16 [filter] => raw ) [136] => WP_Term Object ( [term_id] => 241 [name] => Warehousing [slug] => 159 [term_group] => 0 [term_taxonomy_id] => 241 [taxonomy] => category [description] => [parent] => 210 [count] => 1 [filter] => raw ) [137] => WP_Term Object ( [term_id] => 240 [name] => Websites [slug] => 115 [term_group] => 0 [term_taxonomy_id] => 240 [taxonomy] => category [description] => [parent] => 210 [count] => 1 [filter] => raw ) [138] => WP_Term Object ( [term_id] => 83 [name] => White Papers [slug] => 52 [term_group] => 0 [term_taxonomy_id] => 83 [taxonomy] => category [description] => [parent] => 4 [count] => 6 [filter] => raw ) [139] => WP_Term Object ( [term_id] => 137 [name] => Window Maker FAQ [slug] => 41 [term_group] => 0 [term_taxonomy_id] => 137 [taxonomy] => category [description] => [parent] => 128 [count] => 21 [filter] => raw ) [140] => WP_Term Object ( [term_id] => 227 [name] => Work Order [slug] => 136 [term_group] => 0 [term_taxonomy_id] => 227 [taxonomy] => category [description] => [parent] => 225 [count] => 10 [filter] => raw ) [141] => WP_Term Object ( [term_id] => 138 [name] => Zero Flow Capillary FAQ [slug] => 42 [term_group] => 0 [term_taxonomy_id] => 138 [taxonomy] => category [description] => [parent] => 128 [count] => 20 [filter] => raw ) ) POST QUERY: POST QUERY RESULTS
AppNotes Cogent RP C8
ARTICLES, APPNOTES, FAQ
INTERNAL ONLY – CONFIDENTIAL
ONLINE TOOLS
SALES POLICIES

May 22, 2012
Bill Ciccone

KB Home

AppNotes Cogent Bidentate C18 / AppNotes Library / AppNotes Normal Phase / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Choose Reversed or Normal Phase Selectivity on the Same C18 Column

In the Chromatogram (A) below, a Reversed Phase Method Strategy is used:

Loratadine in 100% Tetrahydrofuran (THF) elutes with the Solvent Front.
With 70:30 DI Water / Tetrahydrofuran (THF), the Selectivity for this Compound is Reversed Phase, which is based on polarity of the compounds.

In the Chromatogram (B) below, a Normal Phase Method Strategy is used:

At 75:25 Hexane / Tetrahydrofuran (THF) Selectivity for Loratadine is Normal Phase and based on Functional Groups of the Compound.

Implications:
Different Selectivity based on Polarity or Functional Groups of an Analyte can be chosen with the same HPLC Column: Cogent Bidentate C18. An advantages is that these Columns do not suffer from the normal difficulties experienced with traditional HPLC Columns for example Cyano, Pentafluorophenyl (F5) or Amino, when converting from Aqueous to Organic Mobile Phases and back again; continual conversions do not damage or alter the Cogent Bidentate C18 Columns.

Loratadine Chromatogram
Loratadine Chemical Structure
Peak:
Loratadine

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-7.5
Dimensions: 75 x 4.6mm
Mobile Phase Solvents:
—A. DI Water 0.1% Trifluoroacetic Acid (TFA)
—B. THF (Tetrahydrofuran)
—C. Hexane
Flow rate: 1mL / minute
Detection: UV @ 255nm

Notes: These method strategies have many uses:
—1. Orthogonal Peak Identification
—2. Normal Phase separations on a C18 phase to achieve separations that are not possible without using ion-pair reagents in Reversed Phase.
—3. Single Column to scout for optimal methods in both Normal and Reversed Phase mode.
—4. ARP / ANP – Analytical Chromatography.
—5. NP – Preparative Chromatography.

Attachment

No 14 Orthogonal HPLC Method Development pdf 0.2 Mb Download File

If you can’t find what you are looking for, email us

Contact Us