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        • High pressure PEEK.
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          • ETFE – Tefzel.
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        • Tubing Tools.
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  • About Us
    • Company Profile
    • Why Do Business with Us
    • Policies and Procedures
    • Holiday Schedule
  • Products
    • Categories
      • ARE-Applied Research Products
      • Autosampler Vials, Caps, Inserts & Accessories
        • ** Caps **
          • Caps – AQR™ brand screw & snap.
          • Crimp caps.
          • Headspace caps.
          • Push caps.
          • Screw caps.
          • Septa only – for caps.
          • Snap caps.
        • ** Vials & inserts **
          • *Screw top vials.*
          • Crimp top vials – serum finish.
          • EPA style vials & caps.
          • Headspace vials.
          • Inserts for low volume samples.
          • Push top vials
          • RSA-Pro X™ vial kits & inserts.
          • RSA™ low volume vials & inserts.
          • RSA™ vials – screw & snap tops.
          • Specialty vials.
          • U-2D™ Micro-sample management.
        • *** Vial & cap kits ***
          • Crimp top vial & cap kits.
          • Push top vial & cap kits.
          • RSA™ vial & cap kits
          • RSA™ vials, caps & inserts.
          • Screw top vial & cap kits.
          • Snap top vial & cap kits.
        • Accessories for vials.
        • Storage vials, caps & vial boxes.
        • The Vial Centrifuge™.
      • Bottles for Chromatography
        • Mobile phase bottles for HPLC – safety coated.
        • Mobile phase bottles for HPLC – non-coated.
        • Solvent bottle caps & accessories.
      • Capillary Electrophoresis
        • Capillaries for CE.
        • Miscellaneous CE items.
        • Window Maker™.
      • Di-Ad HPLC Column Connectors
      • Filters – Mobile Phase Solvent
        • Membrane filters discs.
        • Mobile phase inlet filters.
        • Vacuum solvent filter degassers.
      • FlexiTubes – Space Saving PEEK HPLC Tubing
      • Headspace Vials & Caps
        • Caps for headspace vials.
        • Vials for headspace.
      • HPLC Column Hardware
        • Column packing / slurry reservoirs.
        • Frits – SS & Titanium
        • PEEK column hardware.
      • HPLC Columns
        • Amide
        • Amino
          • Cogent HPS™ Amino
          • Cogent MicroBee™ Amino
          • Cogent NP™ Amino
        • Bidentate C18™
        • Bidentate C8™
        • C12
        • C18
          • C18 Similars™
            • Cogent Hyper-Similars™ C18
            • Cogent MicroBee™ C18
            • Cogent Nova-Similars™ C18
            • Cogent Zx Similars™ C18
          • Cogent Axis™ C18
          • Cogent HPS™ C18
          • Cogent RP C18 ABX™
          • Cogent RP™ C18
          • Cogent RP™ C18 PE
        • C30
        • C4
        • C8
          • C8 Similars™
            • Cogent Hyper-Similars™ C8
            • Cogent MicroBee™ C8
            • Cogent Nova Similars™ C8
            • Cogent Zx Similars™ C8
          • Cogent HPS™ C8
          • Cogent RP™ C8
        • Cogent Column Filters
        • Column Couplers
        • Cyano
          • Cogent Axis™ Cyano
          • Cogent HPS™ Cyano
          • Cogent MicroBee™ Cyano
          • Cogent NP™ Cyano
          • Cogent RP™ Cyano
        • Diamond Hydride™
        • Diol
          • Cogent Diol™
          • Cogent NP™ Diol
        • Guard column holders
        • Ion Exchange
          • Cogent IEX SAX™
          • Cogent IEX SCX™
          • Cogent IEX WAX™
          • Cogent IEX WCX™
        • Method Development Kits
          • Metabolomics development kits
          • TYPE-C method development kits
        • Partisil & Partisphere
          • Partisil™
          • Partisphere™
        • PFP Pentafluorophenyl
        • Phenyl
          • Cogent HPS™ Phenyl
          • Cogent RP™ Phenyl
          • Phenyl Similars™
            • Cogent MicroBee™ Phenyl
            • Cogent Nova-Similars™ – Phenyl
        • Phenyl Hexyl
        • Phenyl Hydride™
        • Silica – (un-modified)
          • Cogent HPS™ Silica
          • Cogent MicroBee™silica
          • Cogent NP™ Silica
          • Cogent Silica-C™
        • UDA™ (weak cation exchange)
        • UDC-Cholesterol™
      • Plates & Sealing Mats
      • Qualification Kits & HOX
        • Kits for HPLC.
        • Kits for UV-vis.
      • Sample Loops
      • Syringe Filters – AQ™ Brand
      • Syringes for Chromatography
      • Tools For Chromatography
      • Tubing – Laboratory Grade
        • High pressure PEEK.
        • High pressure SS.
        • Low pressure polymeric.
          • ETFE – Tefzel.
          • FEP – Teflon FEP.
          • PFA – PerFlourAlkoxy.
          • PTFE – Teflon.
        • Tubing Tools.
      • Unions & Adapters – Tubing Connectors
        • Union adapters for tubing.
        • Unions for HPLC tubing.
      • Value and Clearance Items
        • Chrom Syringes – Clearance
        • Filter Sparging Stones – Clearance
        • Metal frits & filter discs – Clearance
        • Miscellaneous – Clearance
    • Specifications of Products
    • Product Literature
  • Fittings & Accessories for HPLC
    • Column end plugs.
    • Nuts & ferrules.
  • Information
    • Application Notes
    • Calculators
    • CE Primer
    • FAQ
    • Glossary
    • HPLC Tips and Tricks
    • MSDS
    • Operating Manuals
    • Request Troubleshooting and Technical Assistance
    • Specifications of Products
    • Technical Information Links
    • USP Listing of Columns
    • Videos
    • White Papers
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    • New Reseller Application Form
    • Report a Problem
    • Request AutoSampler Vial Catalog
    • Request Samples
    • Request Software Demo
    • Technical Assistance Options
  • Resource Center
    • Application Notes by Compounds
    • HPLC Column Information
    • Cogent TYPE-C™ Column Information
    • Frequently Asked Questions??
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    • ARTICLES, APPNOTES, FAQ
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  • What’s New at MicroSolv

Category: TYPE-C in General FAQ

KB Home/ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Columns FAQ / TYPE-C in General FAQ /

How to improve LOD or detection limits in HPLC – Tips & Suggestions

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How to keep HPLC columns free of adsorbed contaminants – How To

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How to keep surface treated frits from biofouling – FAQ

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How to separate phospholipids with HPLC – Tips & Suggestions

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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds – FAQ

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I see a strange saw tooth type of pattern in the UV baseline using my Cogent HPLC column what is it and how can I fix it – FAQ

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Improvement in signal to noise in LCMS with an ANP method – Tips & Suggestions

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Increasing selectivity and resolution for a xylose and ribose method – Tips & Suggestions

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Injection volume suggestions for Cogent TYPE-C HPLC columns – Tips & Suggestions

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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns – FAQ

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Is TYPE-C Silica a high purity support and have a low metal content silica like Type B silica – FAQ

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Method development tip for maleic hydrazide – Tips & Suggestions

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My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material – FAQ

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My method uses an standard type-B silica based HPLC column is there a Cogent TYPE-C column equivalent substitute – FAQ

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Negative Baselines in HPLC at 214nm – Tips & Suggestions

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No need to use a high pH mobile phases for compounds with a high pKa – Tips & Suggestions

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Obtain a flat baseline in a gradient method without using blank subtraction – How to

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Percentage of acid needed in Aqueous Normal Phase ANP methods – Tips & Suggestions

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Policy on verifying a new Cogent HPLC column performance – Tips & Suggestions

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Purge metals from HPLC system using EDTA or medronic acid – How To

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Random shifting of retention times in HPLC chromatograms – Tips & Suggestions

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Sample concentration changes can effect retention time in HPLC – Primer

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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP – FAQ

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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible – FAQ

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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ

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Store and condition Cogent TYPE-C HPLC columns – How To

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Store and maintain Cogent Diamond Hydride HPLC columns – How To

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The common names of HPLC column diameters – Primer

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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase – Tips & Suggestions

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Theoretical plate estimates indicates column condition – Primer

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Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run – Tips & Suggestions

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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source – Tips & Suggestions

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Use Teflon bottles for A and B solvents in negative ion mode LCMS – Primer

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Using Aqueous Normal Phase ANP to your advantage – White Paper

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What are inosine nucleotides – FAQ

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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies – FAQ

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What are some of the advantages of using Cogent TYPE-C prep and semi-prep HPLC columns – FAQ

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What are the metal contents of the silica used in Cogent TYPE-C HPLC columns – FAQ

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What are the uses of PEEK HPLC column hardware – FAQ

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What can cause changing retention times and a peak showing up in my blank with a Silica-C column – FAQ

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What does Post Time mean in an LCMS or HPLC gradient method – FAQ

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What does the term Activity refer to in chromatography – FAQ

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What effect does high injection volume have on column performance in Aqueous Normal Phase ANP HPLC- FAQ

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What is a base deactivated HPLC Column – FAQ

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