The Maximum percent of acid can you use to acidify Cogent TYPE-C HPLC Columns.

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AppNotes Cogent RP C8
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April 14, 2020
jimmy

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions

Generally speaking there is not much need to go above 0.5% acetic or formic acid for most ANP applications. If TFA is used such as in biological applications, the usual limit is about 0.1% for UV.

TFA is generally not recommended for LCMS because of ion suppression it can cause.

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