AppNotes Metabolites – MicroSolv Technology Corporation

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1,4-Benzodiazepines in Urine by ANP – AppNote

1,4-Benzodiazepines from Urine Samples were analyzed using a Cogent Diamond Hydride 2.o HPLC Column successfully after Solid Phase Extraction (SPE). Four available Compounds (shown below) were well retained and separated. The procedure could be used for determination of this class of compounds in urine samples and other body fluids. Peaks: 1. Temazepam 301.0739 m/z [M+H]+ […]

3-Hydroxy-3-Methylglutaric acid, HMG in Urine by HPLC – AppNote

Important Biomarker for Diabetes and HMG Disorder is well retained by HPLC. A selective, specific, and sensitive HPLC method with LCMS Analysis has been developed for the determination of 3-hydroxy-3-methylglutaric acid (a.k.a. Meglutol) in urine samples. The method can be also used in the analysis of plasma samples after precipitation of plasma proteins with Acetonitrile. […]

4’-Aminoacetophenone: Drug Metabolite – AppNote

4’-aminoacetophenone Attachments No 66 Drug Metabolite.pdf 0.2 Mb Download File

A Cannabis Metabolite in Urine Matrix Analyzed With LCMS – AppNote

Retention, Detection and Quantitation of a Cannabis Metabolite from Urine Samples Using the SPE technique in “Sample Preparation” section of this AppNote, successful MS detection of a Cannabis Metabolite using the Cogent Phenyl Hydride™ HPLC Column is described. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile […]

Acetyl-L-Carnitine (ALC) in Plasma – AppNote

Excellent LC-MS method in spiked plasma sample For more detailed application, click here. Attachments No 240 Acetyl-L-Carnitine in Plasma.pdf 0.3 Mb Download File

Amino Acids, Non Derivatized – AppNote

From Synthetic Urine Attachments No 58 Non Derivatized Amino Acids.pdf 0.2 Mb Download File

Analysis of Nucleotides by ANP – AppNote

ATP & AMP separated in Aqueous Normal Phase (ANP) Attachments No 95 Analysis of Nucleotides by ANP.pdf 0.1 Mb Download File

Analysis of Thiopurines – AppNote

Separation using LCMS Gradient Method The Cogent Diamond Hydride Column with gradient elution was used for analysis of Thiopurines. Figure A shows the Peak of 6-TG, which also can be analyzed using Isocratic conditions. Good retention and symmetrical peak shape were obtained under the analysis conditions. Figure B represents two separated Thiopurines (Mono and Di- […]

Analyte Ionization using L-tyrosine – AppNote

L-Tyrosine capsule Attachments No 179 LC-MS Tips for Promoting Analyte Ionization.pdf 0.4 Mb Download File

Anatoxin-A Method Development Strategy for a Polar Compound – AppNote

Using Anatoxin-a Attachments No 142 Method Development Strategy for a Polar Compound.pdf 0.3 Mb Download File

Anatoxin-a, ANTX-A – AppNote

Potent Neurotoxin Attachments No 141 Anatoxin-a ANTX-A.pdf 0.3 Mb Download File

Arginine, Lysine & Histidine, Basic Amino Acids – AppNote

In Synthetic or Human Urine Attachments No 60 Basic Amino Acids.pdf 0.3 Mb Download File

Aspartic Acid & Arginine: Amino Acids – AppNote

Reduced Sample Prep and Handling Attachments No 63 Easy Amino Acid Method.pdf 0.2 Mb Download File

ATP, UDP-Glucose UDP-Galactose Separation from Red Blood Cells – AppNote

Separation of Nucleotides In this application, we present an easy-to-use protocol for sensitive detection and quantification of nucleotides in red blood cell extracts. Retention times were very reproducible with %RSD around 0.4. The desired separation between UDP-Glu and UDP-Gal was achieved, all other nucleotides eluted later and did not interfere with the analysis. ATP represents […]

Atropine determination using a simple gradient – AppNote

No ion pair reagent necessary and still very precise Attachments No 117 Precise Determination of Atropine using a simple gradient.pdf 0.1 Mb Download File

Benzylpiperazine (BZP) – AppNote

Analysis in Hair Samples using LCMS An analytical protocol was developed for analysis of Benzylpiperazine in human hair samples. The elucidation of the chromatographic method was challenging due to the polar nature of BZP. The limits of detection / quantification for this method were determined to be 0.05 ng / mg for Benzylpiperazine in hair […]

Bile Acids from Urine – AppNote

Separation in LCMS using the Diamond Hydride Attachments No 55 Bile Acids from Urine.pdf 0.2 Mb Download File

Cannabis Metabolite in Urine Matrix – AppNote

Cannabis Metabolite in Urine Matrix is well retained on a Cogent Phenyl Hydride HPLC Column. The extraction procedure using an SPE technique allows for successful detection of this Cannabis metabolite with the Cogent Phenyl Hydride Column. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile as the […]

Carisoprodol & Meprobamate – AppNote

Analysis of closely related compounds Attachments No 124 Carisoprodol and Meprobamate.pdf 0.2 Mb Download File

Carotenoids Analyzed by SFC – AppNote

Separation in HPLC using the Bidentate C18 Attachments No 43 Selectivity & Retention for Carotenoids in SFC.pdf 0.2 Mb Download File

Citric, Aconitic & Maleic acids by ANP – AppNote

Low Molecular Weight Organic Acids Attachments No 64 Citric Acid by ANP.pdf 0.2 Mb Download File

Codeine and Hydrocodone in Blood Analyzed by LCMS – AppNote

LC-MS Separation of isobaric Drugs in Urine Codeine and Hydrocodone which are isobaric drugs (i.e. the same m/z value) were separated using this AppNote. Multiple samples (n=5) were prepared and analyzed. The results showed excellent reproducibility (RSDs < 2.5%). The procedure in this AppNote, after validation, can be used in forensic toxicological laboratories to determine […]

Creatine, Creatinine & Hydroxyproline metabolites – AppNote

Separation in LCMS using the Diamond Hydride Attachments No 49 Metabolites in Urine.pdf 0.2 Mb Download File

Cyanuric acid and Melamine determinations – AppNote

LCMS method Attachments No 93 Simple ANP Method for Determinations of Cyanuric Acid and Melamine.pdf 0.2 Mb Download File

Different Solvent Selectivity Mechanism – AppNote

Polarity or Shape Recognition – YOU CHOOSE! Attachments No 18 Diferent Solvent Selectivity Mechanism.pdf 0.2 Mb Download File

Domoic Acid & Amino Acids in Algae Extracts – AppNote

Low level analysis without analyte derivatization Attachments No 156 Domoic Acid (DA) & Amino Acids in Algae Extracts.pdf 0.1 Mb Download File

Efficiency of ANP v. HILIC Columns – AppNote

HILIC v ANP at High Flow Rate on Standard HPLC Equipment with a Diamond Hydride Column. Attachments No 110 Very High Flow Rate, High Efficiency & Selectivity.pdf 0.3 Mb Download File

Ethanolamine – AppNote

Retention of Hydrophilic Analyte from Plasma Attachments No 210 Ethanolamine.pdf 0.3 Mb Download File

Galactose-1-Phosphate HPLC method – AppNote

Clinical Screening Method Attachments No 191 Galactose-1-Phosphate.pdf 0.3 Mb Download File

Glutamine and Lysine Determination – AppNote

Two Isobaric Amino Acids in Saliva Attachments No 102 Glutamine and Lysine Determination.pdf 0.2 Mb Download File

Glutathione (GSH) Analyzed by LCMS – AppNote

LCMS Method without Derivatization The major benefit of this analysis method for Glutathione which uses the Cogent Diamond Hydride Column and MS detection is that it is performed without derivatization, unlike other LCMS approaches. A GSH signal of MS is stable after repeated analyses. The unique design of the ESI offers efficient supersoft ionization which […]

Glyburide and Metformin – AppNote

Separation of “Highly Polar” and “Non-Polar” Compounds in one isocratic run using Reverse Phase Solvents. Attachments No 24 Glyburide and Metformin.pdf 0.2 Mb Download File

Glyburide and Metformin HPLC method – AppNote

Separation of “Highly Polar” and “Non-Polar” Compounds in one isocratic run Attachments No 08 Glyburide and Metformin.pdf 0.2 Mb Download File

Guanidine by Aqueous Normal Phase – AppNote

Very High Signal To Noise Ratio for Reproducible Results Attachments No 78 Guanidine by Aqueous Normal Phase.pdf 0.2 Mb Download File

Hesperidin Analyzed by HPLC – AppNote

LC-MS compatible method This method for analysis of Hesperidin produces an excellent analyte peak and shows separation from what appears to be two impurity peaks. The gradient method is LC-MS compatible and can be applied to many types of Hesperidin-containing samples such as citrus fruit extracts. The overlay of five runs shown in the figure […]

Histamine and Methylhistamine HPLC method – AppNote

No Derivatization Required Attachments No 197 Histamine and Methylhistamine HPLC method and application note.pdf 0.2 Mb Download File

Hormones Separation – AppNote

with Cogent Bidentate C18™ Capillary LC Column. Attachments No 45 Separation of Hormones.pdf 0.2 Mb Download File

Human Plasma Extract Analyzed by LCMS – AppNote

Urea, Lactodifucotetraose, Trimethylamine, L-carnitine & Choline Using this Method it is possible to analyze Compounds present in a Human Plasma Samples. The mechanism of retention of these polar compounds on the Column used is unknown at this time. It is possible to Retain and Separate steroids and other Compounds based on the Shape of the […]

Hypoxantine, Chenodeoxycholic acid, Betaine & Choline – AppNote

Separation in LCMS using the Diamond Hydride Attachments No 53 Metabolites in Human Urine.pdf 0.2 Mb Download File

Ionization effect of microM addition of Sodium Acetate for Glucose – AppNote

Monosaccharide – Glucose retention in the ANP mode Attachments No 115 Ionization Effect of microM Addition of Sodium Acetate.pdf 0.2 Mb Download File

Isobaric metabolites in urine – AppNote

A simple & reproducible LCMS friendly method Attachments No 88 Determination of Important Isobaric Metabolites in Urine.pdf 0.3 Mb Download File

Isotretinoin in Human Plasma – AppNote

Drug used for treatments of cystic acne, cancer, and keratinization For more detailed application, click here. Attachments No 238 Isotretinoin in Human Plasma.pdf 0.2 Mb

L-a-phenylglycine Antibiotic Analysis – AppNote

Using Cogent Silica-C™ column Attachments No 46 Phenylglycine for Antibiotic Analysis.pdf 0.3 Mb Download File

Leucine & iso Leucine branch chain amino acids – AppNote

Leucine, Isoleucine Attachments No 84 Branched-chain amino acids (BCAAs).pdf 0.1 Mb Download File

LSD and Codeine – AppNote

LCMS Method for Spiked Urine Sample LSD and codeine may be present in Urine samples and methods are needed for their detection in these matrices. The extracted Ion Chromatograms (EICs) for both analytes are shown in the figure and illustrate excellent chromatographic as well as Mass Spectral Separation. The Method in this AppNote produces high […]