1,4-Benzodiazepines in Urine by ANP – AppNote

1,4-Benzodiazepines from Urine Samples were analyzed using a Cogent Diamond Hydride 2.o HPLC Column successfully after Solid Phase Extraction (SPE). Four available Compounds (shown below) were well retained and separated. The procedure could be used for determination of this class of compounds in urine samples and other body fluids. Peaks: 1. Temazepam 301.0739 m/z [M+H]+ […]

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3-Hydroxy-3-Methylglutaric acid, HMG in Urine by HPLC – AppNote

Important Biomarker for Diabetes and HMG Disorder is well retained by HPLC. A selective, specific, and sensitive HPLC method with LCMS Analysis has been developed for the determination of 3-hydroxy-3-methylglutaric acid (a.k.a. Meglutol) in urine samples. The method can be also used in the analysis of plasma samples after precipitation of plasma proteins with Acetonitrile. […]

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A Cannabis Metabolite in Urine Matrix Analyzed With LCMS – AppNote

Retention, Detection and Quantitation of a Cannabis Metabolite from Urine Samples  Using the SPE technique in “Sample Preparation” section of this AppNote, successful MS detection of a Cannabis Metabolite using the Cogent Phenyl Hydride™ HPLC Column is described. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile […]

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Analysis of Thiopurines – AppNote

Separation using LCMS Gradient Method The Cogent Diamond Hydride Column with gradient elution was used for analysis of Thiopurines. Figure A shows the Peak of 6-TG, which also can be analyzed using Isocratic conditions. Good retention and symmetrical peak shape were obtained under the analysis conditions. Figure B represents two separated Thiopurines (Mono and Di- […]

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ATP, UDP-Glucose UDP-Galactose Separation from Red Blood Cells – AppNote

Separation of Nucleotides  In this application, we present an easy-to-use protocol for sensitive detection and quantification of nucleotides in red blood cell extracts.  Retention times were very reproducible with %RSD around 0.4. The desired separation between UDP-Glu and UDP-Gal was achieved, all other nucleotides eluted later and did not interfere with the analysis. ATP represents […]

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Benzylpiperazine (BZP) – AppNote

Analysis in Hair Samples using LCMS An analytical protocol was developed for analysis of Benzylpiperazine in human hair samples. The elucidation of the chromatographic method was challenging due to the polar nature of BZP. The limits of detection / quantification for this method were determined to be 0.05 ng / mg for Benzylpiperazine in hair […]

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Cannabis Metabolite in Urine Matrix – AppNote

Cannabis Metabolite in Urine Matrix is well retained on a Cogent Phenyl Hydride HPLC Column. The extraction procedure using an SPE technique allows for successful detection of this Cannabis metabolite with the Cogent Phenyl Hydride Column. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile as the […]

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Codeine and Hydrocodone in Blood Analyzed by LCMS – AppNote

LC-MS Separation of isobaric Drugs in Urine Codeine and Hydrocodone which are isobaric drugs (i.e. the same m/z value) were separated using this AppNote. Multiple samples (n=5) were prepared and analyzed. The results showed excellent reproducibility (RSDs < 2.5%). The procedure in this AppNote, after validation, can be used in forensic toxicological laboratories to determine […]

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Glutathione (GSH) Analyzed by LCMS – AppNote

LCMS Method without Derivatization The major benefit of this analysis method for Glutathione which uses the Cogent Diamond Hydride Column and MS detection is that it is performed without derivatization, unlike other LCMS approaches. A GSH signal of MS is stable after repeated analyses. The unique design of the ESI offers efficient supersoft ionization which […]

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Hesperidin Analyzed by HPLC – AppNote

LC-MS compatible method This method for analysis of Hesperidin produces an excellent analyte peak and shows separation from what appears to be two impurity peaks. The gradient method is LC-MS compatible and can be applied to many types of Hesperidin-containing samples such as citrus fruit extracts. The overlay of five runs shown in the figure […]

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Human Plasma Extract Analyzed by LCMS – AppNote

 Urea, Lactodifucotetraose, Trimethylamine, L-carnitine & Choline Using this Method it is possible to analyze Compounds present in a Human Plasma Samples. The mechanism of retention of these polar compounds on the Column used is unknown at this time. It is possible to Retain and Separate steroids and other Compounds based on the Shape of the […]

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LSD and Codeine – AppNote

LCMS Method for Spiked Urine Sample LSD and codeine may be present in Urine samples and methods are needed for their detection in these matrices. The extracted Ion Chromatograms (EICs) for both analytes are shown in the figure and illustrate excellent chromatographic as well as Mass Spectral Separation. The Method in this AppNote produces high […]

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