Analyzing Bisphenol A in Carbonless Paper – AppNote

Bisphenol A (BPA) is a synthetic compound used in a variety of consumer products, such as CDs, water bottles, and water pipe linings. Despite having many uses in these products, there has been some concern in recent years that exposure to BPA may have adverse health implications. BPA is believed to act as an endocrine […]

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Bisphenol A, BPA an HPLC Method – AppNote

Toxic Substance Found in Consumer Products Analyzed by HPLC Bisphenol A (BPA) is a challenging compound for analysis by HPLC. Monitoring of this environmental and health toxicant is a necessary process for surveillance as well as risk assessment. As shown in the chromatograms below (5 overlaid), a Cogent Bidentate C8 Column produces Peak shapes that […]

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Bromhexine HPLC Assay Method – AppNote

I am currently using the following Method for analysis of Bromhexine. What Column and/or alternative Method Conditions can you Suggest? Mobile phase: Potassium di-hydrogen phosphate buffer(take Potassium di-hydrogen phosphate 1.0g, add 900ml DI Water, then change pH to 7.0 with 0.5mol/L NaOH solution, and dilution it to 1000ml by DI Water )-acetonitrile(20:80), Column temperature: 40C, Wavelength:245nm […]

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Chlorpheniramine Maleate Organic Impurities Analyzed by HPLC – AppNote

Separation demonstrated in real commercial formulation extracts Use of the Cogent Bidentate C8™ Column allows for high resolution baseline separation of six specified impurities of Chlorpheniramine Maleate (Fig. A). Fig. B shows how the method can be applied to a real-world formulation, spiked with the N-oxide impurity to demonstrate resolution from the API peak. Currently, […]

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Dehydroepiandrosterone (DHEA) Capsule Separation by HPLC – AppNote

Excellent separation from matrix excipients Pharmaceutical formulations may contain a number of excipients that can potentially interfere with a chromatographic analysis. However, using the Cogent Bidentate C8 Column, the DHEA peak is well-resolved from the others, the latter of which all elute as a large peak in the solvent front portion of the run. The […]

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Download an Analysis of Harmful Substances in Dietary Supplements – AppNote

Dietary Supplements have had, in notable cases, ingredients added to them that have been implicated in serious health issues and even death. Two such pernicious compounds are Aegeline and 1,3-Dimethylamylamine. These two can be readily separated from the dietary supplement matrix using the Cogent Bidentate C18 2.o™   and Cogent Diamond Hydride™ Columns by LCMS […]

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Hormone Replacement HPLC Methods – AppNote

Chromatographic Separation between Various Components in Hormone Replacement Formulations. Often, these formulations have multiple active ingredients present, therefore HPLC is an appropriate technique in obtaining the necessary specificity for a quantitative assay. The Cogent Bidentate C8 and C18™ Columns retain the hormones by conventional Reversed Phase HPLC, while the UDC-Cholesterol™ can also differentiate among the […]

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Mometasone Furoate in Topical Cream Analysis by HPLC – AppNote

Separation of Prodrug from Acid Hydrolysis Product This method shows the separation of an ester prodrug, Mometasone Furoate, from another compound formed during acid hydrolysis. This peak is quite possibly the active form, Mometasone. The two chromatograms show the non-degraded extract (Figure A) and an acid hydrolysis extract (Figure B). Hydrolysis of the ester group […]

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