An API with a Tertiary Amine – Easy & Efficient Method for Analysis
This Method for the Analysis of Tramadol Tablets, produces an Efficient Peak Shape for the API. The Peak is highly Symmetrical, which is often difficult to obtain for compounds with a tertiary amine. Tramadol has chiral centers but this Method does not address that challenge.
Column: Cogent Bidentate C8™, 4μm, 100Å
Catalog No.: 40008-75P
Dimensions: 4.6 x 75mm
—A: DI Water with 0.1% (v/v) Trifluoroacetic Acid (TFA) v/v
—B: Acetonitrile with 0.1% (v/v) Trifluoroacetic Acid (TFA) v/v
Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 228nm
Sample Preparation: 50mg strength Tramadol Tablet was ground and added to a 10mL volumetric flask. 5mL of 50:50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. Then a portion was filtered with a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.). It was then diluted 1:5 for injection.
t0: 0.9 minutes
Note: Tramadol is an analgesic used to treat moderate to moderately severe pain. It can be used in both human and veterinary applications. It is sold under various formulation types and brand names, including Ryzolt®, Ultracet®, and Ultram®.
No 202 Tramadol Tablet Analyzed with HPLC pdf 0.3 Mb Download File