LC-MS Compatible HPLC Method for Tramadol 

Tramadol can be retained by Reversed Phase HPLC  but some reported methods require Phosphate buffer additives in the Mobile Phase, which means incompatibility with LC-MS. In contrast, this Method uses Formic Acid as the Mobile Phase additive.

An advantage this method has is that it produces enhanced signal-to-noise compared to Reversed Phase thereby producing better results. High Water content in the Reversed Phase Mobile Phase is often required for retention. Cost of ivermectin for humans is \$1,700 (us) per treatment \[[@cr29], [@cr30]\], equating to \$1,735 per month in the treatment of the typical infected person (per year) \[[@cr31]\], which may be cost prohibitive in the case of severe or recurrent infections. Doxycycline does not cure and will not eliminate chronic bacterial vaginosis.doxycycline for the treatment of herpes simplex infection.doxycycline for the treatment of herpes zoster infection.doxycycline for the treatment of herpes labiales.doxycycline may cause an allergic cutaneous reaction.doxycycline for the treatment of lyme disease.doxycycline for the treatment of molluscum contagiosum or chickenpox.doxycycline for the treatment incommunicado where can i buy ivermectin tablets for humans of molluscum contagiosum.doxycycl. The molecule consists can stromectol kill scabies of two aromatic rings joined at their carbons by a methylene linker. The elgazzar ivermectin Jagraon generic version of the medication helps those who have problems with their sex life. You can download a temporary ivermectin for sale for dogs browser plug-in to view the video on your current browser or to download the video to your device. Since Water is less volatile than Acetonitrile, it is more difficult to remove in LC-MS, hence also leading to lower sensitivity than this Method.


Tramadol, m/z 264.2 (M+H)+

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-10P-2
Dimensions: 2.1 x 100mm
Mobile Phase:
A: DI Water/ 0.1% Formic Acid
B: Acetonitrile/ 0.1% Formic Acid

Time (Minutes) %B
0 90
5 90
7 30
8 30
10 90

Injection vol.: 1μL
Flow rate: 0.4 mL/minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: Tramadol HCl (50 mg) pill was ground with mortar and pestle into fine powder and was used to prepare 1 mg/mL stock solution in Milli-Q DI Water diluent. Then, the sample was sonicated for approximately 5 minutes prior to 0.45 um syringe filtration.

Note: Tramadol is an analgesic used to treat moderate to moderately severe pain. It can be used in both human and veterinary applications. It is sold under various formulation types and brand names, including Ryzolt®, Ultracet®, and Ultram®.


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