10 Phenolic Acids Separated with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of phenolic compounds in the sample with Reversed Phase HPLC. The retention and separation of all nine available standards was possible using the method […]

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5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

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A Cannabis Metabolite in Urine Matrix Analyzed With LCMS – AppNote

Retention, Detection and Quantitation of a Cannabis Metabolite from Urine Samples  Using the SPE technique in “Sample Preparation” section of this AppNote, successful MS detection of a Cannabis Metabolite using the Cogent Phenyl Hydride™ HPLC Column is described. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile […]

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Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse Analysis – AppNote

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain in a chromatographic separation. Second, a good number of […]

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An Analysis of Local Anesthetic Dyclonine HCL by HPLC – AppNote

A Reproducible Method for Detection of Dyclonine Hydrochloride using Reversed Phase HPLC A rapid, sensitive, and reproducible method has been developed for detection of Dyclonine HCL. The data below, (overlay of 20 injections) illustrates how the compound can be adequately retained and detected using HPLC, with both excellent peak shape and run-to-run precision. Using a […]

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Analysis of MDMA in Plasma Samples by LCMS – AppNote

MDMA – Analyzed by HPLC with MS Under the described conditions, MDMA was retained and eluted as a symmetrical peak. The sensitivity of the method is very good and  comparable to that reported with GC-MS detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise quantification. The method can be […]

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Cannabis Metabolite in Urine Matrix – AppNote

Cannabis Metabolite in Urine Matrix is well retained on a Cogent Phenyl Hydride HPLC Column. The extraction procedure using an SPE technique allows for successful detection of this Cannabis metabolite with the Cogent Phenyl Hydride Column. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile as the […]

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Comparison of Extracts in Jaboticaba Fruit Powder by HPLC with LCMS – AppNote

Phenolic Compounds Separated by HPLC with LCMS. This simple Analytical Method was used to determine the content of very potent compounds present in Jaboticaba extracts and quantified some its phenolic contents in fermented/non-fermented fruit; estimated by using standard addition. It is important to know the quantitation of compounds before and after fermentation to optimize the […]

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Coricidin HBP Night® Tablet – AppNote

Acetaminophen, Dextromethorphan, and Chlorpheniramine This method demonstrates the potential of the Cogent Phenyl Hydride 2.o™ Column for analysis of amine-containing analytes in a real formulation. Chlorpheniramine and Dextromethorphan can often be problematic in terms of peak tailing due to the tertiary amines, but this column and method produces excellent peak shapes for both compounds in this […]

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Cyanidin-3-O-Glucoside Analysis in Fruit- AppNote

Analysis in Fruit and Vegetable Extracts In this analysis of proprietary fruit & vegetable extracts, the presence of Cyanidin-3-O-Glucoside was confirmed in three out of four extracts. The peaks were symmetrical and retained beyond the dead volume. The method after validation can be used to quality control of commercial fruit extracts as well in studies […]

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Determination of Phenolic Compound by LCMS – AppNote

Compounds in Olive Leaves Extract A commercial olive extract was analyzed using the Cogent Phenyl Hydride Column. Only one oleuropein peak was detected and it was symmetrical and well retained. The results were reproducible (%RSD = 0.2 for retention times). According to the literature [1] the extract from olive leaves should contain additional compounds. To […]

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Glycosidic Constituents of Mesquite Flour Analysis with LCMS – AppNote

Observed in Mesquite flour extracts, these compounds are Glycosidic Flavones and Anthocyanins. The Glycoside moieties impart polar characteristics to the analytes, which makes them amenable to retention by the Aqueous Normal Phase (ANP) retention mode. In this application note, the Cogent Phenyl Hydride Column was used to obtain Chromatographic Separation. The same column can be […]

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Ketorolac Separation by HPLC and LCMS – AppNote

Analgesic agent There is a need for analysis of the Active Pharmaceutical Ingredient (API) in Ketorolac Tablet formulations in order to evaluate two different extraction protocols. The use of Ethanol as the extraction solvent was explored initially but DI Water was observed to produce superior extraction efficiency. The chromatograms show how the compound can be […]

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Mycotoxins – AppNote

The detection of mycotoxins has been an area of increasing focus in ensuring human health due to their high toxicity even at low concentrations. These chemicals are produced by various species of mold, including Aspergillus Penicillium, Paecilomyces, and Fusarium types. Exposure can come from ingestion of infested food products or, in the case of fungal […]

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Peonidin-3-Glucoside Analyzed by LCMS – AppNote

LC-MS method for Anthocyanin- Fruit or Vegetable Extracts In this analysis, the presence of Peonidin-3-O–Glucoside was confirmed in two out of four extracts. The peaks were symmetrical and retained beyond the dead volume. Due to the MS detection and comparison of the retention time, it was possible to find the peak for Peonidin-3-O-Glucoside among many […]

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Rice Extract Analyzed by LCMS – AppNote

Content of Phenolic Compounds in Rice Determined by LCMS When commercial rice extracts were analyzed, only 6’-OFeruloylsucrose at 3.01 minutes (peak not shown for the clarity of the (chromatogram) was found in this particular rice extract sample. Next, the sample was spiked with the standards according to the literature referenced below [1]. The Cogent Phenyl […]

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Spiked Hesperidin Extract Analyzed by LCMS – AppNote

Eriocotrin, Hesperidin, Naringin, Diosmin In this method, the Hesperidin extract was spiked with three glycosidic flavonoids. The Cogent Phenyl Hydride Column was used to separate the main component of the extract (Hesperidin) and three standards. The MS detection helped to distinguish between the Naringin peak of the standard and a small impurity peak (peak marked […]

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Vanillic Acid and 6′-O-Feruloylsucrose Analyzed by LCMS – AppNote

Rice Extract Sample Containing Vanillic Acid and Feruloylsucrose When commercial Rice Extracts were analyzed, only Vanillic Acid (Peak 1) was found in one out of 3 Extracts and 6′-O-Feruloylsucrose (peak 2) was found in all 3 extracts. The results were confirmed by analyzing spiked Rice Extract samples. The analysis was done in a single run […]

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β-Carotene Capsule Analyzed by HPLC – AppNote

Separation from other matrix peaks Beta-carotene may be taken as a dietary supplement in capsule form. In this case, a wide variety of Matrix Peaks were observed in the chromatographic data. It is possible that some of these peaks are various isomers of all-trans ß-carotene or other similar carotenes. In any case, resolution was obtained […]

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