Difficulties with wavelength accuracy readings for HPLC PQ – Blog

Evaluation of wavelength accuracy on older, less sensitive detectors can be a little difficult at times. Getting bubbles in the flow cell is a problem and we recommend that you pull the Wavelength Calibration solution through the flow cell, then disconnect the syringe, and allow the system to rest, and keep the inlet/outlet tubing ends above the flow cell […]

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How do I determine wavelength accuracy below 230nm for HPLC instruments?

Holmium Oxide is an internationally recognized wavelength calibration primary standard, supported by NIST and other standards organizations. It provides 14 official absorbance bands ranging from 241 nm to 641 nm. Caffeine is considered a secondary standard, with two absorbance bands at 205 nm and 273 nm. Caffeine is also widely used as a wavelength and […]

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How do I validate the Excel spreadsheet that comes with the HSQ™ Kit?

There is no need for the end-user to perform any validation of HSQ Kit™ Excel spreadsheet. The document is fully validated by the factory. A certificate of validation can be provided upon request if needed (see below attached document). The scope of the validation encompasses the design of the spreadsheet, that its calculations are accurate and perform […]

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Validation Protocol for the PQ Kit Template- Tips & Suggestions

The Validation Protocol in the attachment is intended to Document and Validate the MicroSolv HPLC PQ Kit Excel Template used for HPLC Qualifications. The Excel file given for the latest version of the Performance Qualification is for 97-2003 Microsoft Excel Worksheet, but this is also compatible with Excel 2010.  For operating in Excel 2010, you […]

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What is the difference between Verification, Validation and Qualification?

In an LCGC Magazine article by Drs. Ira Krull and Michael Swartz they reference the International Organization for Standardization (ISO) as follows: “Validation is confirmation, through the provision of objective evidence that the requirements for a specific intended use or application have been fulfilled” whereas Verification is confirmation, through the provision of objective evidence that specified requirements have […]

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