What is the difference between related compounds and related substances in pharmaceutical HPLC – FAQ Read More →
What is the difference between weak and strong cation exchange for Ion Exchange columns – FAQ Read More →
What is the effect of extra column volume for different HPLC instruments on the same method – FAQ Read More →
What is the maximum concentration of ammonium fluoride to use in a mobile phase to avoid column damage – Tips & Suggestions Read More →
What is the maximum loading capacity mass injection volume that can be used for a semi prep Cogent TYPE-C HPLC column – FAQ Read More →
What is the maximum salt concentration to use with Cogent TYPE-C HPLC columns with HPLC or LCMS – FAQ Read More →
What is the most effective way to clean HPLC columns after or before use – Tips & Suggestions Read More →
What is the pilot length or dimension X depth for end fittings uses to connect HPLC columns – FAQ Read More →
What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods – FAQ Read More →
What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time – FAQ Read More →
What special care should I take to protect my HPLC columns when I am equilibrating them – FAQ Read More →
What type of tubing do I use to connect the guard column holder to analytical columns – FAQ Read More →
What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column – FAQ Read More →
When I run gradients on my HPLC and switch from one gradient to another my baseline shows as negative and if I auto zero it becomes positive for the remainder of the run but when I start again it is negative – Primer Read More →
Which acid or base additive should I choose for positive or negative ion mode in LC-MS – FAQ Read More →
Which column do you suggest for Cocaine & metabolite Ecognin separation – Tips & Suggestions Read More →
Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents – FAQ Read More →
Why are environmental conditions such as temperature relative humidity that are not specified on the CofA for the UV Calibration Kit – FAQ Read More →
Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC – FAQ Read More →
Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column – FAQ Read More →