Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC – FAQ

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ARTICLES, APPNOTES, FAQ (9438)
- AppNotes Library (1910)
  - AppNotes Alternate Generic Methods (28)
  - AppNotes ANP/HILIC Methods (199)
  - AppNotes Listed by Column (492)
    
    AppNotes Cogent Biphenyl (1)
    
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    AppNotes Cogent RP C8 (8)
    
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    AppNotes TYPE-C Columns (397)
    
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    AppNotes Cogent Bidentate C18 (96)
    
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    AppNotes Cogent Bidentate C8 300 (5)
    
    AppNotes Cogent Diamond Hydride (181)
    
    AppNotes Cogent Diol (7)
    
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    AppNotes Cogent UDC Cholesterol (19)
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    AppNotes Proteins Peptides (9)
  - AppNotes Normal Phase (4)
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- Autosampler Vials Information (323)
  - Headspace Vials & Caps (27)
  - HPLC & GC Standard Vials Caps & Inserts (66)
  - Plastic Vials (28)
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- Chromatography Accessories & Supplies Information (473)
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    Beta Tool Information (2)
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- Frequently Asked Questions?? (3461)
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    MicroSolv Autosampler Vials FAQ (118)
    
    RSA Vials FAQ (112)
    
    U-2D Sample MGT FAQ (57)
    
    Vials & Caps General FAQ (147)
  - CE and CZE FAQ (179)
    
    Buffers for CE FAQ (18)
    
    Controlled Flow Capillary FAQ (33)
    
    Window Maker FAQ (26)
    
    Zero Flow Capillary FAQ (30)
  - Equipment FAQ (98)
    
    Knitted Reactor Coils (14)
    
    Tips Liquid Handling (7)
  - Filtration FAQ (177)
    
    Chrom Syringes FAQ (7)
    
    Cogent Column Filters – FAQ (24)
    
    Filter Degasser FAQ (23)
    
    Syringe Filters FAQ (44)
  - Fittings & Tubing for HPLC FAQ (120)
  - HPLC Columns FAQ (820)
    
    2.o™ HPLC Columns FAQ (99)
    
    Amide FAQ (8)
    
    Bidentate C18 FAQ (26)
    
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    Column Hardware FAQ (74)
    
    Diamond Hydride FAQ (84)
    
    Diol FAQ (6)
    
    Guard Columns FAQ (27)
    
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April 14, 2020
Bill Ciccone

ANP Aqueous Normal Phase Articles / Frequently Asked Questions?? / HPLC Tips and Suggestions / TYPE-C Column Information

The highest polarity is generally found in ionized compounds due to the fixed charge on the compound.

Therefore in Aqueous Normal Phase ANP HPLC, strongest retention is attained with the highest polarity, when the compounds are fully ionized.

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