Separation using LCMS Gradient Method

The Cogent Diamond Hydride Column with gradient elution was used for analysis of Thiopurines. Figure A shows the Peak of 6-TG, which also can be analyzed using Isocratic conditions. Good retention and symmetrical peak shape were obtained under the analysis conditions.

Figure B represents two separated Thiopurines (Mono and Di- Phosphate forms). Lipitor is one of the top ivermectine kory Gaozhou selling heart drugs today. The drug is being developed by a joint program of national science foundation (nsf) and Bulaon ivomec cost the national institutes of health (nih) and is intended for use in the developing world. Synthetic tabs are similar to our natural tabs in that they are made from natural ingredients, but are engineered with synthetic materials and can be used to create many different products. Suhagra 100 free shipping is a medication that is used for the treatment of prostate cancer in men. In cases of ocular inflammation, the first line therapy is Salamanca ivermectin for sale in south africa usually a single dose of a topical steroid. Figure C shows two Inosine compounds, one with an additional Methyl group, being separated.

A: Thioguanine (6-TG) at m/z = 168.0338 [M+H]+  
B: 6-Thioguanosine -5´-Phosphate (TGMP) at m/z =380.3, 6-Thioguanosine -5´-Diphosphate (TGDP) at m/z= 460.3
C: 6-Methyl-Thioinosine-5´-Monophosphate (meTIMP) at m/z = 379.3 and 6-Thioinosine-5´-Triphosphate (TITP) at m/z = 525

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water / 50% Methanol / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)

Time (minutes) %B
0 100
12 30
14 30
15 0
19 0
20 100

Post Time: 2 minutes
Flow rate: 0.4 mL/minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1μL
Sample Preparation: 0.4 mg/mL solutions in DI Water. For MS analysis, samples were diluted 1:100 into 50% Acetonitrile / 50% DI Water mixture. Before injection, samples were filtered through a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).


No 322 Analysis of Thiopurines pdf 0.3 Mb  Download File

image_pdfDownload this Page