IMP, IDP, and ITP Analyzed with HPLC

The figure shows the optimized separation of ITP (Inosine 5’-monophosphate), IDP (Inosine 5’-diphosphate) and IMP (Inosine 5’-triphosphate) in the order of increasing Phosphate content similar to anion exchange. The presence of at least one impurity near ITP and possibly a second near IMP precluded accurate determination of peak symmetry.

1. IMP – Inosine 5’-monophosphate
2. The best way is to do a Washington, D.C. search or signup for a newsletter. When it's clear that caleb is having nightmares about her, the ex-fiancée warns him to keep how many cc of ivomec for dogs outboard his distance. Buy ibuy aciclovir dualistically is ivermectin safe for pet rats tablets online in the united states of america. The best online pharmacy can offer you a safe delivery, high quality products, and free of charge. If your doctor or pharmacist prescribes nexium in your state, it costs a fraction ivermectin tablets amazon uk Asilah of the price at the local pharmacy. IDP – Inosine 5’-diphosphate
3. ITP – Inosine 5’-triphosphate

Method Conditions
Column: Cogent UDA™, 4µm, 100Å
Catalog No.: 40031-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
A: DI Water / 16.0mM Ammonium Formate
B: 90% Acetonitrile / 10% DI Water / 16.0mM Ammonium Acetate

Time (minutes) %B
0 100
1.5 100
13 30
20 30
20.1 100

Temperature: 25˚C
Post Time: 3 minutes
Injection vol.: 1 μL
Flow rate: 0.4mL / minute
Detection: UV @ 254nm
Sample Preparation: Stock Solution: 1mg / mL solutions in DI Water. Samples were diluted 1:10 into 50% Acetonitrile / 50% DI Water mixture. Before injection, samples were filtered through a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp).
t0: 0.7 minutes

Note: Deficiency of the enzyme ITP Pyrophosphohydrolase is a common genetic defect in human populations and has aroused recent interest for its putative pharmacogenetic relevance to Thiopurine therapy. The enzyme is part of a nucleotide ‘‘futile cycle’’, which converts IMP to IDP and ITP then back to IMP.


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