Ticagrelor Analyzed with HPLC – AppNote

A Reproducible Method for Detection of this Blood Clot Preventative  A rapid, sensitive, and Reproducible Method has been developed for analysis of Ticagrelor. The data below, (overlay of 5 chromatograms ) illustrates how the compound can be adequately Retained and detected using a simple Gradient in Reversed Phase HPLC.  The Method demonstrates good Peak Shape […]

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Epigallocatechin Gallate Analyzed with HPLC – AppNote

A Reliable Method for a EGCG Analysis This Epigallocatechin Gallate (EGCG) Assay produces Run to Run consistency and Precision with Retention Time RSD values of 0.02%.  This is a sensitive HPLC-UV Method that may be used for quantification of EGCG in food and beverage formulations. Peak: Epigallocatechin Gallate  Method Conditions: Column: Cogent Zx Similars C18, […]

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Deoxycholic Acid Analyzed with an ELSD- AppNote

An Analysis of a Bile Acid This simple Isocratic Method shows excellent Peak shape and Reproducibility as shown in the five injection overlay below. As the shape of the molecule is very similar to Steroids, the UDC-Cholesterol™ Column was selected for this Method founded on its ability to retain compounds based on shape, hydrophobicity, and […]

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Deoxycholic Acid Assay Analyzed with HPLC – AppNote

A Reliable Method for a Bile Acid This Deoxycholic Acid Assay produces Run to Run consistency and Precision with Retention Time RSD values of 0.1%. A sensitive HPLC-UV Method that may be used for quantification in raw material and liquid pharmaceutical formulation is presented. Figure 1. Full Chromatogram Figure 2. Magnified Chromatogram to show peak […]

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Tazobactam USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method of Tazobactam This USP Tazobactam Assay was easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met. This Application Note demonstrates a good Alternate Column for your Tazobactam […]

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Retention of a Tricyclic Amine Analyzed with ELSD- AppNote

Memantine Hydrochloride, a Tricyclic Amine As this compound lacks Chromophores, it typically requires Derivitization for use in UV detection.  In this Method, we retain excellent peak shape without the need for these pre-column derivatization steps. This compound can also cause issues with Peak Tailing in typical Reversed Phase Columns due to the strong adsorption of […]

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Ifosfamide Assay Analyzed with HPLC – AppNote

A Reliable Method for an Antineoplastic Chemotherapeutic Drug This Ifosfamide Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.8%. This Method shows reliability for analysis of this Pyrimidine analog that is an antineoplastic anti-metabolite. Peak: Ifosfamide Method Conditions: Column: Cogent RP C18™, 5um, 100Å Catalog […]

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Fluorouracil Assay Analyzed with HPLC – AppNote

A Reliable Method for a Chemotherapeutic Drug This Fluorouracil Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.1%. This Method shows reliability for analysis of this Pyrimidine Analog that is an anti-neoplastic anti-metabolite. Peak: Fluorouracil Method Conditions: Column: Cogent RP C18™, 5um, 100Å Catalog No.: […]

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Gemcitabine Assay Analyzed with HPLC – AppNote

A Reliable Method for a Chemotherapeutic Drug This Gemcitabine Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.4%. This Method shows consistency and reliability for analysis of this Nucleoside Chemotherapeutic. Peak: Gemcitabine Method Conditions: Column: Cogent RP C8™, 5um, 150Å Catalog No.: 68508-25P Dimensions: 4.6mm […]

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Desloratadine Assay with HPLC – AppNote

Alternate L1 Column used for HPLC Method of Desloratadine This Desloratadine Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values below 0.3%. This demonstrates a good alternate Column for your Desloratadine […]

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Fluphenazine HCl USP Tablet Assay with HPLC – AppNote

Alternate L7 Column used for the USP Assay Method of Fluphenazine HCl Tablets This USP Fluphenazine HCl Tablet Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.2%. This demonstrates […]

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Hydrocortisone USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method of Hydrocortisone This USP Hydrocortisone Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.5%. This demonstrates a good alternate Column […]

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RSA-Pro X Improves Quantitation Precision with HPLC – AppNote

RSA-Pro X™ – Hydrolytically Stable Glass Vials & Low Volume Glass Inserts Silanol groups (Si-OH) on the surface of glass has been known to cause not only empirical quantitative loss but also inaccurate quantitation, vial to vial and run to run over time.  Silanol sites are very reactive and can produce among other things, imprecise […]

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Does the HSQ Kit Qualify Quaternary HPLC Pumps? – FAQ

Yes, the HSQ Kit™ will work on many types of pumps; from single, binary, and also quaternary pumps.  For Gradient Qualification Tests, the base method is called “GRDAB” for “Gradient, pump A/B*”, where the “*” designates that the B fluid circuit is “visualized” with the addition of the Gradient Visualization Solution (GVS). For ternary or […]

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Lipophilic Peptides Analyzed with HPLC – AppNote

Simple Gradient for Non-Polar Peptides  In this Method, we demonstrate the Retention of several Hydrophobic Peptides without the use of a typical Reversed Phase Column. Peak: 1. Gly-Tyr ( Glycyl-L-tyrosine ) 2. Val-Tyr-Val ( Valyltyrosylvaline ) 3. Lignocaine hcl 20 mgs odt, lignocaine hcl 10 attentively mgs odt. And after reading this article, http://diezelelectric.com/98053-buy-gabapin-55264/ you […]

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Cytarabine Assay with HPLC- AppNote

A Reliable Assay for Detection of a Chemotherapeutic   Cytarabine belongs to the category of chemotherapy called antimetabolites. Antimetabolites are very similar to normal substances within the cell. When these substances are incorporated into the cellular metabolism, they are unable to divide. Lignocaine hcl 20 mgs odt, lignocaine hcl 10 attentively mgs odt. And after […]

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4-(Methylamino)butyric Acid Analyzed with HPLC- AppNote

A gamma-Aminobutyric Acid (GABA) Derivative  This challenging polar compound is easily retained in this simple Method where repeatability of the data is excellent with %RSD<0.1 for the Retention Times. Peak: 4-(Methylamino)butyric Acid Method Conditions: Column: Cogent Diamond Hydride™, 4μm, 100Å Catalog No.: 70000-15P-2 Dimensions: 4.6 x 150mm Mobile Phase: —A: DI Water with 0.1% Formic […]

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Retention of a Polypeptide Analyzed with HPLC- AppNote

Semaglutide, a Human Glucagon-like Peptide This simple isocratic Method demonstrates good Retention of a Hydrophilic Peptide where Retention is increased in Aqueous conditions.   Peak: Semaglutide Method Conditions: Column: Cogent Diamond Hydride™, 4μm, 100Å. Catalog No.: 70000-75 Dimensions: 4.6 x 75mm Mobile Phase: 60:30 DI Water with 0.1% Formic Acid / Acetonitrile. Injection vol.: 1µL […]

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Amoxicillin USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method for Amoxicillin This USP Amoxicillin Assay is performed and a five injection overlay shows Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.3%. This demonstrates a good alternate Column for […]

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Sulfonamide Antibiotics Analyzed with HPLC- AppNote

Everyday Reliable & Precise Method This Method offers great Resolution of a mixture of four Sulfa drugs.  These Antibiotics are commonly used in both veterinary medicine and also employed to fight disease in honey bees.  The importance of quantification is vital, as residues are often found in honey samples and are of concern to customers […]

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Triton X-100 Assay with HPLC- AppNote

A Sensitive Assay for Detection of a Non-ionic Surfactant  Triton X-100 is used in the sample purification steps of various materials as well as for cleaning apparatus in pharmaceutical manufacturing.  Due to the toxic nature of Triton X-100, a sensitive method for its detection and quantification is very important to identify any residual amounts. This simple […]

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Moxifloxacin Hydrochloride Analyzed with HPLC – AppNote

Simple and Precise Retention of a Polar Antibiotic  Moxifloxacin Hydrochloride requires the addition of buffers as well as ion pair reagents to aid in retention on regular Reversed Phase Columns per the current USP Method. In this simple isocratic Method, we retain this polar compound with good Run-to-Run Precision.  (%RSD = 0.22, SD below 0.004.)  This […]

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Tetracycline USP Assay with HPLC- AppNote

USP Method for Tetracycline This USP Tetracycline Assay is performed and a five injection overlay shows run to run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.4%. This demonstrates a good Alternate Column for your USP Method.   Peak: Tetracycline Method Conditions: […]

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Topiramate, Retention of a Sulfonate Drug by LCMS – AppNote

Retention of the Sulfonate Drug Topiramate The structure of Topiramate leads us to think due to the Amino group and negative Log P value of -0.8, it will not be retained in Reversed Phase HPLC. However, in this Method, the compound is well retained. Peak: Topiramate 340.1062 m/z (M + H)+ Method Conditions: Column: Cogent […]

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Dexamethasone and Prednisone Assay with HPLC- AppNote

Analysis of Corticosteroids This simple isocratic Method demonstrates good separation of two similar synthetic Glucocorticoids.  This analysis for corticosteroids may serve well for quantitation in pharmaceutical preparations.  This Method would easily translate to LCMS. Peak: 1. Prednisone 2. Dexamethasone  Method Conditions: Column: Cogent Bidentate C18™, 4µm, 100Å Catalog No.: 40018-75 Dimensions: 4.6 x 75mm Mobile […]

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Felodipine Extended-Release Tablets USP Assay – AppNote

USP Method for Felodipine ER Tablets This method presents the USP Felodipine Extended-Release Tablet assay using the Cogent RP C18. As shown in the chromatogram with a five injection overlay, the peak efficiency is superb and USP peak tailing guidelines are easily met with the Cogent RP C18™ column.  This demonstrates a great alternative Column […]

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Cyclobenzaprine Hydrochloride USP Assay – AppNote

This method demonstrates the USP Cyclobenzaprine Hydrochloride Extended-Release Capsules Assay. As shown in the Chromatograms, the peak efficiency is superb and USP peak tailing guidelines are easily met with the Cogent RP C18 5μm column. This demonstrates a great alternative column for easy plug and play and this UPS Assay Method. Method Conditions: Column: Cogent […]

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Amlodipine, Valsartan, and Hydrochlorothiazide Tablet USP Assay – AppNote

This AppNote demonstrates the USP Method for Amlodipine, Valsartan, and Hydrochlorothiazide Tablets working with the Cogent RP C18. As shown in the Chromatograms, the Peak efficiency is superb and USP Peak tailing guidelines are easily met with the Column. This demonstrates a great alternative Column for easy plug and play with this USP Method. Method […]

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Dimethylolpropionic Acid – AppNote

Hydrophilic acid retention on the Cogent Diamond Hydride™  Dimethylolpropionic Acid is a unique diol molecule with both hydroxyl and carboxyl functionality and presents several challenges in chromatographic analysis. Traditional reversed phase mode may not be viable due to the high hydrophilicity of this organic acid. Also, by lacking chromophores it can be difficult to detect […]

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Analysis of a Local Anesthetic Lidocaine, Aminoethylamide – AppNote

Method Development for a Local Anesthetic (A Case Study) An analyst was using a Cogent Diamond Hydride™ Column for evaluating Lidocaine methods and although they had good retention of the compound, they did not meet the required 0.3 – 0.5 AU when 0.1 mg/mL Lidocaine sample was injected for both 2μL and 10μL injection volumes. […]

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Psychoactive Compounds Analysis in Cannabinoids – AppNote

Cannabinoid Separation Method Due to the number of expanding products of homeopathic extracts from Hemp, there is an equally increasing necessity to accurately analyze and separate the numerous compounds from these natural samples. While Cannabidiol has been extracted for multiple natural remedies, there is also numerous unwanted psychoactive constituents and related compounds that may also […]

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Morphine Sulfate Tablet HPLC Method Transferred to Near UHPLC – AppNote

Standard 4um Particle Size Transferred to 2.o™ This application illustrates how methods developed using the 4µm Cogent Diamond Hydride Columns may be adapted for Cogent Diamond Hydride 2.o™ phases. Morphine shows slightly higher retention on the 2.o™ Column (average 7.311 min vs. 5.588 min). Lignocaine hcl 20 mgs odt, lignocaine hcl 10 attentively mgs odt. […]

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Ketorolac Separation with LCMS – AppNote

Analgesic agent There is a need for analysis of the Active Pharmaceutical Ingredient (API) in Ketorolac Tablet formulations in order to evaluate two different extraction protocols. The use of Ethanol as the extraction solvent was explored initially but DI Water was observed to produce superior extraction efficiency. The chromatograms show how the compound can be […]

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Nitrofurantoin Capsule Analyzed with HPLC – AppNote

Isocratic Separation of API from USP internal standard The USP assay method for the active pharmaceutical ingredient (API) found in Nitrofurantoin Capsules, uses a Phosphate Buffer and is not compatible with Mass Spectrometry. In this AppNote, using the Cogent Bidentate C18 Column, Formic Acid is used as the Mobile Phase additive, as such, the method […]

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Bi-Est® Method Transfer – AppNote

4μm to Near-UHPLC – Separation of Hormones: Estriol, Estradiol, and Progesterone Separation of three components of a hormone replacement formulation is demonstrated in this Application Note using the Cogent Bidentate C18 2.o™ Column. The two figures demonstrate how comparable retention can be obtained for both the near-UHPLC column as well as the standard 4μm Column, […]

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Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. Lignocaine […]

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Resveratrol Capsule – AppNote

Isocratic Separation of Isomers In this easy isocratic method, the cis and trans isomers of Resveratrol are separated using a near-UHPLC stationary phase. The Cogent Bidentate C18 2.o™ Column produces excellent efficiency for both analyte peaks. The sample used here is an actual capsule formulation, demonstrating the suitability of the column for real-world samples. Lignocaine […]

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Metaldehyde in Slug Pellets Analyzed by LCMS – AppNote

Pesticide Formulation As Metaldehyde is a non-UV absorbing compound, other detection methods needed to be investigated besides conventional UV-HPLC. LC-MS was found to be a well-suited analysis by searching for the EIC corresponding to the [M+H]+ ion. Good retention and peak shape were observed for this analyte using the Cogent Bidentate C18 2.o™ Column. Peak: […]

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Coricidin HBP Night® Tablet – AppNote

Acetaminophen, Dextromethorphan, and Chlorpheniramine This Method demonstrates the potential of the Cogent Phenyl Hydride 2.o™ Column for analysis of amine-containing analytes in a real formulation. Chlorpheniramine and Dextromethorphan can often be problematic in terms of peak tailing due to the tertiary amines, but this column and method produces excellent peak shapes for both compounds in this […]

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Analysis of Isobaric Opioids by LCMS – AppNote

Separation of Isobaric Drugs Morphine and Hydromorphone are Isobaric Drugs (the same m/z value) that can be separated using the Cogent Bidentate C18 2.o™ Column. Multiple samples (n=5) were prepared and analyzed. The results showed great reproducibility (RSDs < 3%). Lignocaine hcl 20 mgs odt, lignocaine hcl 10 attentively mgs odt. And after reading this […]

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Doxycycline and Methacycline Analyzed with HPLC – AppNote

Simple Isocratic Method for API and Main Impurity Formulations of Doxycycline may contain a Methacycline Impurity.  As such, it is crucial for analytical methods to be able to separate these two peaks. Using this simple isocratic Method, the baseline resolution is obtained for this pair. Peaks: 1. Methacycline 2. Lignocaine hcl 20 mgs odt, lignocaine […]

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Peonidin-3-Glucoside Analyzed by LCMS – AppNote

LC-MS method for Anthocyanin- Fruit or Vegetable Extracts In this analysis, the presence of Peonidin-3-O–Glucoside was confirmed in two out of four extracts. The peaks were symmetrical and retained beyond the dead volume. Due to the MS detection and comparison of the retention time, it was possible to find the peak for Peonidin-3-O-Glucoside among many […]

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Glycosidic Flavonoids Analyzed with HPLC – AppNote

Separation of Diosmin, Eriocitrin, and Naringin In this method, three Glycosidic flavonoid standards are separated with good resolution. The Cogent Bidentate C18 2.o™ Column produces high efficiency peaks with reproducible retention (see figure overlay). Separation is also observed for a small impurity peak, eluting between peaks 1 and 2. Lignocaine hcl 20 mgs odt, lignocaine […]

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Limonin in Orange Juice Analyzed by LC-MS – AppNote

Separation from Matrix Peaks with Reproducible Results Analysis of Limonin in Spiked Orange Juice was performed using a simple isocratic method with MS detection. Analysis at 471.2 m/z allowed for detection of only the Limonin peak without interference of the juice matrix. When the Filtered, Unspiked Orange Juice was injected, a small peak for Limonin […]

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Oral OTC Anesthetic Analyzed with HPLC – AppNote

Isocratic HPLC Method for Phenol in Oral OTC Products This Method shows how an assay of Phenol can be readily performed with a simple isocratic Mobile Phase. Phenol is the active ingredient in this common Over-The-Counter oral spray solution, which is intended for treatment of sore throat. A number of inactive ingredients are present in […]

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Docusate Sodium Analyzed with HPLC – AppNote

A Simple Gradient with Good Separation    The official USP assay method for Docusate Sodium tablets calls for a Mobile Phase of 50% 7mM Ammonium Acetate / 50% Acetonitrile at 40°C. However, retention for the API and the System Suitability compound Methyl Paraben were found to be low using these conditions. This Method not only […]

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Hydroxytryptophan (5-HTP) Analyzed with HPLC- AppNote

Separation from Vitamin B6 Pyridoxine (Vitamin B6) is often added to formulations of 5-Hydroxytryptophan (5-HTP), as it assists in the conversion of 5- HTP to Serotonin. Therefore, it is of interest to separate these two compounds for an HPLC assay Method of this combination formulation. The Method shown provides good resolution between the two peaks […]

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