How can I fix split peaks with high back pressure in HPLC analyses – FAQ

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ARTICLES, APPNOTES, FAQ
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April 14, 2020
Bill Ciccone

How To... Information / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Trouble Shooting Suggestions

This is most likely due to “channeling” of the flow pattern inside the column.

The packed bed uniformity has likely been disrupted and the split peaks and high pressure are indicative of this. In this case, the column will need to be replaced.

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