How can I Fix an Issue of Split Peaks and a High Column Pressure in HPLC Analyses? – FAQ

collapsCat options: Array ( [title] => Article Categories [showPostCount] => 0 [inExclude] => exclude [inExcludeCats] => default-title,uncategorized,featured-articles,180,1651,ce-accessories [showPosts] => 0 [showPages] => 0 [linkToCat] => 1 [olderThan] => 0 [excludeAll] => 0 [catSortOrder] => ASC [catSort] => catName [postSortOrder] => ASC [postSort] => postTitle [expand] => 4 [defaultExpand] => [debug] => 1 [postTitleLength] => 0 [catfeed] => none [taxonomy] => category [post_type] => post [postDateAppend] => after [postDateFormat] => m/d [showPostDate] => 0 [useCookies] => 0 [postsBeforeCats] => 0 [expandCatPost] => 1 [showEmptyCat] => 1 [showTopLevel] => 1 [useAjax] => 0 [customExpand] => [customCollapse] => [style] => kubrick [accordion] => 1 [title_link] => [addMisc] => 0 [addMiscTitle] => [number] => 2 [includeCatArray] => Array ( ) [expandSym] => [collapseSym] => ) postsToExclude: Array ( ) CATEGORY QUERY RESULTS Array ( [0] => WP_Term Object ( [term_id] => 165 [name] => 2.o™ HPLC Columns FAQ [slug] => 127 [term_group] => 0 [term_taxonomy_id] => 165 [taxonomy] => category [description] => [parent] => 153 [count] => 9 [filter] => raw ) [1] => WP_Term Object ( [term_id] => 211 [name] => Accounting - AP/AR Department [slug] => 116 [term_group] => 0 [term_taxonomy_id] => 211 [taxonomy] => category [description] => [parent] => 210 [count] => 22 [filter] => raw ) [2] => WP_Term Object ( [term_id] => 221 [name] => Accounting Dept. Forms [slug] => 124 [term_group] => 0 [term_taxonomy_id] => 221 [taxonomy] => category [description] => [parent] => 211 [count] => 5 [filter] => raw ) [3] => WP_Term Object ( [term_id] => 229 [name] => Accounts Receivable [slug] => 147 [term_group] => 0 [term_taxonomy_id] => 229 [taxonomy] => category [description] => [parent] => 225 [count] => 2 [filter] => raw ) [4] => WP_Term Object ( [term_id] => 154 [name] => Amide FAQ [slug] => 168 [term_group] => 0 [term_taxonomy_id] => 154 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [5] => WP_Term Object ( [term_id] => 214 [name] => App Notes Internal, USP & Archived [slug] => 142 [term_group] => 0 [term_taxonomy_id] => 214 [taxonomy] => category [description] => [parent] => 5 [count] => 30 [filter] => raw ) [6] => WP_Term Object ( [term_id] => 106 [name] => AppNotes AgroChem [slug] => 83 [term_group] => 0 [term_taxonomy_id] => 106 [taxonomy] => category [description] => [parent] => 105 [count] => 11 [filter] => raw ) [7] => WP_Term Object ( [term_id] => 265 [name] => AppNotes Alternate Generic Methods [slug] => appnotes-usp-methods [term_group] => 0 [term_taxonomy_id] => 265 [taxonomy] => category [description] => [parent] => 14 [count] => 9 [filter] => raw ) [8] => WP_Term Object ( [term_id] => 113 [name] => AppNotes ANP/HILIC Methods [slug] => 138 [term_group] => 0 [term_taxonomy_id] => 113 [taxonomy] => category [description] => [parent] => 14 [count] => 191 [filter] => raw ) [9] => WP_Term Object ( [term_id] => 107 [name] => AppNotes Chemicals/Energy [slug] => 86 [term_group] => 0 [term_taxonomy_id] => 107 [taxonomy] => category [description] => [parent] => 105 [count] => 24 [filter] => raw ) [10] => WP_Term Object ( [term_id] => 94 [name] => AppNotes Cogent Amide [slug] => 167 [term_group] => 0 [term_taxonomy_id] => 94 [taxonomy] => category [description] => [parent] => 84 [count] => 9 [filter] => raw ) [11] => WP_Term Object ( [term_id] => 97 [name] => AppNotes Cogent Bidentate C18 [slug] => 92 [term_group] => 0 [term_taxonomy_id] => 97 [taxonomy] => category [description] => [parent] => 84 [count] => 92 [filter] => raw ) [12] => WP_Term Object ( [term_id] => 57 [name] => AppNotes Cogent Bidentate C8 [slug] => 97 [term_group] => 0 [term_taxonomy_id] => 57 [taxonomy] => category [description] => [parent] => 84 [count] => 16 [filter] => raw ) [13] => WP_Term Object ( [term_id] => 96 [name] => AppNotes Cogent Bidentate C8 300 [slug] => 100 [term_group] => 0 [term_taxonomy_id] => 96 [taxonomy] => category [description] => [parent] => 84 [count] => 3 [filter] => raw ) [14] => WP_Term Object ( [term_id] => 264 [name] => AppNotes Cogent Biphenyl [slug] => biphenyl-appnotes [term_group] => 0 [term_taxonomy_id] => 264 [taxonomy] => category [description] => Application Notes using the Cogent Biphenyl HPLC Columns. [parent] => 15 [count] => 0 [filter] => raw ) [15] => WP_Term Object ( [term_id] => 98 [name] => AppNotes Cogent Diamond Hydride [slug] => 90 [term_group] => 0 [term_taxonomy_id] => 98 [taxonomy] => category [description] => [parent] => 84 [count] => 166 [filter] => raw ) [16] => WP_Term Object ( [term_id] => 99 [name] => AppNotes Cogent Diol [slug] => 146 [term_group] => 0 [term_taxonomy_id] => 99 [taxonomy] => category [description] => [parent] => 84 [count] => 7 [filter] => raw ) [17] => WP_Term Object ( [term_id] => 160 [name] => AppNotes Cogent HPS [slug] => 135 [term_group] => 0 [term_taxonomy_id] => 160 [taxonomy] => category [description] => [parent] => 15 [count] => 4 [filter] => raw ) [18] => WP_Term Object ( [term_id] => 261 [name] => AppNotes Cogent Phenyl Hexyl [slug] => appnotes-cogent-phenyl-hexyl [term_group] => 0 [term_taxonomy_id] => 261 [taxonomy] => category [description] => [parent] => 15 [count] => 1 [filter] => raw ) [19] => WP_Term Object ( [term_id] => 100 [name] => AppNotes Cogent Phenyl Hydride [slug] => 93 [term_group] => 0 [term_taxonomy_id] => 100 [taxonomy] => category [description] => [parent] => 84 [count] => 38 [filter] => raw ) [20] => WP_Term Object ( [term_id] => 262 [name] => AppNotes Cogent RP C8 [slug] => appnotes-cogent-rp-c8 [term_group] => 0 [term_taxonomy_id] => 262 [taxonomy] => category [description] => [parent] => 15 [count] => 3 [filter] => raw ) [21] => WP_Term Object ( [term_id] => 101 [name] => AppNotes Cogent Silica-C [slug] => 98 [term_group] => 0 [term_taxonomy_id] => 101 [taxonomy] => category [description] => [parent] => 84 [count] => 6 [filter] => raw ) [22] => WP_Term Object ( [term_id] => 102 [name] => AppNotes Cogent UDA [slug] => 99 [term_group] => 0 [term_taxonomy_id] => 102 [taxonomy] => category [description] => [parent] => 84 [count] => 7 [filter] => raw ) [23] => WP_Term Object ( [term_id] => 103 [name] => AppNotes Cogent UDC Cholesterol [slug] => 94 [term_group] => 0 [term_taxonomy_id] => 103 [taxonomy] => category [description] => [parent] => 84 [count] => 17 [filter] => raw ) [24] => WP_Term Object ( [term_id] => 108 [name] => AppNotes Food and Beverages [slug] => 84 [term_group] => 0 [term_taxonomy_id] => 108 [taxonomy] => category [description] => [parent] => 105 [count] => 71 [filter] => raw ) [25] => WP_Term Object ( [term_id] => 14 [name] => AppNotes Library [slug] => 63 [term_group] => 0 [term_taxonomy_id] => 14 [taxonomy] => category [description] => [parent] => 4 [count] => 379 [filter] => raw ) [26] => WP_Term Object ( [term_id] => 15 [name] => AppNotes Listed by Column [slug] => 91 [term_group] => 0 [term_taxonomy_id] => 15 [taxonomy] => category [description] => [parent] => 14 [count] => 25 [filter] => raw ) [27] => WP_Term Object ( [term_id] => 104 [name] => AppNotes Listed by Compound [slug] => 96 [term_group] => 0 [term_taxonomy_id] => 104 [taxonomy] => category [description] => [parent] => 14 [count] => 1 [filter] => raw ) [28] => WP_Term Object ( [term_id] => 105 [name] => AppNotes Listed by Discipline [slug] => 95 [term_group] => 0 [term_taxonomy_id] => 105 [taxonomy] => category [description] => [parent] => 14 [count] => 36 [filter] => raw ) [29] => WP_Term Object ( [term_id] => 110 [name] => AppNotes Metabolites [slug] => 85 [term_group] => 0 [term_taxonomy_id] => 110 [taxonomy] => category [description] => [parent] => 105 [count] => 75 [filter] => raw ) [30] => WP_Term Object ( [term_id] => 258 [name] => AppNotes Normal Phase [slug] => appnotes-normal-phase [term_group] => 0 [term_taxonomy_id] => 258 [taxonomy] => category [description] => [parent] => 14 [count] => 3 [filter] => raw ) [31] => WP_Term Object ( [term_id] => 109 [name] => AppNotes Pharmaceuticals [slug] => 82 [term_group] => 0 [term_taxonomy_id] => 109 [taxonomy] => category [description] => [parent] => 105 [count] => 196 [filter] => raw ) [32] => WP_Term Object ( [term_id] => 111 [name] => AppNotes Proteins Peptides [slug] => 87 [term_group] => 0 [term_taxonomy_id] => 111 [taxonomy] => category [description] => [parent] => 105 [count] => 8 [filter] => raw ) [33] => WP_Term Object ( [term_id] => 114 [name] => AppNotes Reversed Phase Methods [slug] => 140 [term_group] => 0 [term_taxonomy_id] => 114 [taxonomy] => category [description] => [parent] => 14 [count] => 161 [filter] => raw ) [34] => WP_Term Object ( [term_id] => 17 [name] => AppNotes RP C18 [slug] => 181 [term_group] => 0 [term_taxonomy_id] => 17 [taxonomy] => category [description] => [parent] => 15 [count] => 12 [filter] => raw ) [35] => WP_Term Object ( [term_id] => 18 [name] => AppNotes RSA Vials [slug] => rsa-glass-vial-applications [term_group] => 0 [term_taxonomy_id] => 18 [taxonomy] => category [description] => [parent] => 14 [count] => 4 [filter] => raw ) [36] => WP_Term Object ( [term_id] => 84 [name] => AppNotes TYPE-C Columns [slug] => cogent-type-c-silica-columns [term_group] => 0 [term_taxonomy_id] => 84 [taxonomy] => category [description] => [parent] => 15 [count] => 1 [filter] => raw ) [37] => WP_Term Object ( [term_id] => 4 [name] => ARTICLES, APPNOTES, FAQ [slug] => 46 [term_group] => 0 [term_taxonomy_id] => 4 [taxonomy] => category [description] => [parent] => 0 [count] => 1201 [filter] => raw ) [38] => WP_Term Object ( [term_id] => 120 [name] => Autosampler Vials FAQ [slug] => 9 [term_group] => 0 [term_taxonomy_id] => 120 [taxonomy] => category [description] => [parent] => 119 [count] => 24 [filter] => raw ) [39] => WP_Term Object ( [term_id] => 250 [name] => Autosampler Vials Information [slug] => autosampler-vial-tips [term_group] => 0 [term_taxonomy_id] => 250 [taxonomy] => category [description] => [parent] => 4 [count] => 124 [filter] => raw ) [40] => WP_Term Object ( [term_id] => 257 [name] => Autosampler Vials Internal [slug] => autosampler-vials [term_group] => 0 [term_taxonomy_id] => 257 [taxonomy] => category [description] => [parent] => 5 [count] => 1 [filter] => raw ) [41] => WP_Term Object ( [term_id] => 156 [name] => Bidentate C18 FAQ [slug] => 22 [term_group] => 0 [term_taxonomy_id] => 156 [taxonomy] => category [description] => [parent] => 153 [count] => 8 [filter] => raw ) [42] => WP_Term Object ( [term_id] => 155 [name] => Bidentate C8 FAQ [slug] => 23 [term_group] => 0 [term_taxonomy_id] => 155 [taxonomy] => category [description] => [parent] => 153 [count] => 3 [filter] => raw ) [43] => WP_Term Object ( [term_id] => 256 [name] => Buffers Discontinued Internal [slug] => buffers-discontinued [term_group] => 0 [term_taxonomy_id] => 256 [taxonomy] => category [description] => [parent] => 7 [count] => 1 [filter] => raw ) [44] => WP_Term Object ( [term_id] => 129 [name] => Buffers for CE FAQ [slug] => 25 [term_group] => 0 [term_taxonomy_id] => 129 [taxonomy] => category [description] => [parent] => 128 [count] => 5 [filter] => raw ) [45] => WP_Term Object ( [term_id] => 208 [name] => Capillary Electrophoresis Information [slug] => 61 [term_group] => 0 [term_taxonomy_id] => 208 [taxonomy] => category [description] => [parent] => 4 [count] => 31 [filter] => raw ) [47] => WP_Term Object ( [term_id] => 128 [name] => CE and CZE FAQ [slug] => 13 [term_group] => 0 [term_taxonomy_id] => 128 [taxonomy] => category [description] => [parent] => 119 [count] => 9 [filter] => raw ) [48] => WP_Term Object ( [term_id] => 255 [name] => CElixir Products [slug] => celixir-products [term_group] => 0 [term_taxonomy_id] => 255 [taxonomy] => category [description] => [parent] => 7 [count] => 112 [filter] => raw ) [50] => WP_Term Object ( [term_id] => 252 [name] => Chrom Syringes FAQ [slug] => chrom-syringes-faq [term_group] => 0 [term_taxonomy_id] => 252 [taxonomy] => category [description] => [parent] => 147 [count] => 5 [filter] => raw ) [51] => WP_Term Object ( [term_id] => 212 [name] => Click To Chat Internal [slug] => 155 [term_group] => 0 [term_taxonomy_id] => 212 [taxonomy] => category [description] => [parent] => 5 [count] => 5 [filter] => raw ) [52] => WP_Term Object ( [term_id] => 148 [name] => Cogent Column Filters - FAQ [slug] => 37 [term_group] => 0 [term_taxonomy_id] => 148 [taxonomy] => category [description] => This is for the inline or in line PEEK filters that are designed to protect HPLC columns of all brands from unwanted particuatles. [parent] => 147 [count] => 11 [filter] => raw ) [53] => WP_Term Object ( [term_id] => 207 [name] => Cogent HPS FAQ [slug] => 34 [term_group] => 0 [term_taxonomy_id] => 207 [taxonomy] => category [description] => [parent] => 253 [count] => 6 [filter] => raw ) [54] => WP_Term Object ( [term_id] => 167 [name] => Column Hardware FAQ [slug] => 137 [term_group] => 0 [term_taxonomy_id] => 167 [taxonomy] => category [description] => [parent] => 153 [count] => 17 [filter] => raw ) [55] => WP_Term Object ( [term_id] => 135 [name] => Controlled Flow Capillary FAQ [slug] => 32 [term_group] => 0 [term_taxonomy_id] => 135 [taxonomy] => category [description] => [parent] => 128 [count] => 17 [filter] => raw ) [56] => WP_Term Object ( [term_id] => 249 [name] => Cornerstone Scientific Internal [slug] => cornerstone-scientific [term_group] => 0 [term_taxonomy_id] => 249 [taxonomy] => category [description] => [parent] => 5 [count] => 4 [filter] => raw ) [57] => WP_Term Object ( [term_id] => 127 [name] => Crimp Top Vials & Cap FAQ [slug] => 184 [term_group] => 0 [term_taxonomy_id] => 127 [taxonomy] => category [description] => [parent] => 120 [count] => 8 [filter] => raw ) [58] => WP_Term Object ( [term_id] => 222 [name] => Customer Care Department [slug] => 114 [term_group] => 0 [term_taxonomy_id] => 222 [taxonomy] => category [description] => [parent] => 210 [count] => 2 [filter] => raw ) [60] => WP_Term Object ( [term_id] => 157 [name] => Diamond Hydride FAQ [slug] => cogent-diamond-hydride [term_group] => 0 [term_taxonomy_id] => 157 [taxonomy] => category [description] => [parent] => 153 [count] => 42 [filter] => raw ) [61] => WP_Term Object ( [term_id] => 158 [name] => Diol FAQ [slug] => 145 [term_group] => 0 [term_taxonomy_id] => 158 [taxonomy] => category [description] => [parent] => 153 [count] => 1 [filter] => raw ) [62] => WP_Term Object ( [term_id] => 7 [name] => Discontinued Products Internal [slug] => discontinued-products [term_group] => 0 [term_taxonomy_id] => 7 [taxonomy] => category [description] => [parent] => 5 [count] => 16 [filter] => raw ) [63] => WP_Term Object ( [term_id] => 263 [name] => Dursan Surfaces [slug] => dursan-surfaces [term_group] => 0 [term_taxonomy_id] => 263 [taxonomy] => category [description] => [parent] => 77 [count] => 4 [filter] => raw ) [64] => WP_Term Object ( [term_id] => 216 [name] => Employee Cell Phone Internal [slug] => 131 [term_group] => 0 [term_taxonomy_id] => 216 [taxonomy] => category [description] => [parent] => 5 [count] => 1 [filter] => raw ) [65] => WP_Term Object ( [term_id] => 219 [name] => Employee Manual Internal [slug] => 103 [term_group] => 0 [term_taxonomy_id] => 219 [taxonomy] => category [description] => [parent] => 5 [count] => 12 [filter] => raw ) [66] => WP_Term Object ( [term_id] => 139 [name] => Equipment FAQ [slug] => 17 [term_group] => 0 [term_taxonomy_id] => 139 [taxonomy] => category [description] => [parent] => 119 [count] => 8 [filter] => raw ) [67] => WP_Term Object ( [term_id] => 140 [name] => Filter Degasser FAQ [slug] => 20 [term_group] => 0 [term_taxonomy_id] => 140 [taxonomy] => category [description] => [parent] => 147 [count] => 10 [filter] => raw ) [68] => WP_Term Object ( [term_id] => 147 [name] => Filtration FAQ [slug] => 14 [term_group] => 0 [term_taxonomy_id] => 147 [taxonomy] => category [description] => [parent] => 119 [count] => 4 [filter] => raw ) [69] => WP_Term Object ( [term_id] => 220 [name] => Financial Reporting [slug] => 117 [term_group] => 0 [term_taxonomy_id] => 220 [taxonomy] => category [description] => [parent] => 211 [count] => 1 [filter] => raw ) [70] => WP_Term Object ( [term_id] => 152 [name] => Fittings & Tubing for HPLC FAQ [slug] => 12 [term_group] => 0 [term_taxonomy_id] => 152 [taxonomy] => category [description] => [parent] => 119 [count] => 43 [filter] => raw ) [71] => WP_Term Object ( [term_id] => 119 [name] => Frequently Asked Questions?? [slug] => 8 [term_group] => 0 [term_taxonomy_id] => 119 [taxonomy] => category [description] => [parent] => 4 [count] => 3 [filter] => raw ) [72] => WP_Term Object ( [term_id] => 69 [name] => General Information [slug] => 141 [term_group] => 0 [term_taxonomy_id] => 69 [taxonomy] => category [description] => [parent] => 4 [count] => 13 [filter] => raw ) [73] => WP_Term Object ( [term_id] => 223 [name] => General Policies & Procedure Internal [slug] => 109 [term_group] => 0 [term_taxonomy_id] => 223 [taxonomy] => category [description] => [parent] => 210 [count] => 8 [filter] => raw ) [74] => WP_Term Object ( [term_id] => 159 [name] => Guard Columns FAQ [slug] => 81 [term_group] => 0 [term_taxonomy_id] => 159 [taxonomy] => category [description] => [parent] => 153 [count] => 13 [filter] => raw ) [75] => WP_Term Object ( [term_id] => 123 [name] => Headspace Vial FAQ [slug] => 19 [term_group] => 0 [term_taxonomy_id] => 123 [taxonomy] => category [description] => [parent] => 120 [count] => 17 [filter] => raw ) [76] => WP_Term Object ( [term_id] => 70 [name] => How To... [slug] => 76 [term_group] => 0 [term_taxonomy_id] => 70 [taxonomy] => category [description] => [parent] => 4 [count] => 51 [filter] => raw ) [77] => WP_Term Object ( [term_id] => 76 [name] => HPLC Column Information [slug] => 49 [term_group] => 0 [term_taxonomy_id] => 76 [taxonomy] => category [description] => [parent] => 71 [count] => 26 [filter] => raw ) [78] => WP_Term Object ( [term_id] => 153 [name] => HPLC Columns FAQ [slug] => 11 [term_group] => 0 [term_taxonomy_id] => 153 [taxonomy] => category [description] => [parent] => 119 [count] => 10 [filter] => raw ) [79] => WP_Term Object ( [term_id] => 254 [name] => HPLC Columns Old [slug] => hplc-columns-old [term_group] => 0 [term_taxonomy_id] => 254 [taxonomy] => category [description] => [parent] => 7 [count] => 4 [filter] => raw ) [80] => WP_Term Object ( [term_id] => 71 [name] => HPLC Information and Suggestions [slug] => 51 [term_group] => 0 [term_taxonomy_id] => 71 [taxonomy] => category [description] => [parent] => 4 [count] => 15 [filter] => raw ) [81] => WP_Term Object ( [term_id] => 77 [name] => HPLC Tips and Suggestions [slug] => 18 [term_group] => 0 [term_taxonomy_id] => 77 [taxonomy] => category [description] => [parent] => 71 [count] => 340 [filter] => raw ) [82] => WP_Term Object ( [term_id] => 124 [name] => Inserts FAQ [slug] => 158 [term_group] => 0 [term_taxonomy_id] => 124 [taxonomy] => category [description] => [parent] => 120 [count] => 11 [filter] => raw ) [83] => WP_Term Object ( [term_id] => 5 [name] => INTERNAL ONLY - CONFIDENTIAL [slug] => 102 [term_group] => 0 [term_taxonomy_id] => 5 [taxonomy] => category [description] => [parent] => 0 [count] => 62 [filter] => raw ) [84] => WP_Term Object ( [term_id] => 230 [name] => Inventory Module [slug] => 166 [term_group] => 0 [term_taxonomy_id] => 230 [taxonomy] => category [description] => [parent] => 225 [count] => 2 [filter] => raw ) [85] => WP_Term Object ( [term_id] => 215 [name] => IT Computer Warriors Internal [slug] => 156 [term_group] => 0 [term_taxonomy_id] => 215 [taxonomy] => category [description] => [parent] => 5 [count] => 2 [filter] => raw ) [86] => WP_Term Object ( [term_id] => 145 [name] => Knitted Reactor Coils [slug] => 28 [term_group] => 0 [term_taxonomy_id] => 145 [taxonomy] => category [description] => [parent] => 144 [count] => 14 [filter] => raw ) [87] => WP_Term Object ( [term_id] => 206 [name] => Labelled Vials [slug] => 179 [term_group] => 0 [term_taxonomy_id] => 206 [taxonomy] => category [description] => [parent] => 205 [count] => 2 [filter] => raw ) [88] => WP_Term Object ( [term_id] => 224 [name] => Marketing Department [slug] => 119 [term_group] => 0 [term_taxonomy_id] => 224 [taxonomy] => category [description] => [parent] => 210 [count] => 4 [filter] => raw ) [89] => WP_Term Object ( [term_id] => 122 [name] => MicroSolv Autosampler Vials FAQ [slug] => 43 [term_group] => 0 [term_taxonomy_id] => 122 [taxonomy] => category [description] => [parent] => 120 [count] => 9 [filter] => raw ) [90] => WP_Term Object ( [term_id] => 170 [name] => Mobile Phase FAQ [slug] => 72 [term_group] => 0 [term_taxonomy_id] => 170 [taxonomy] => category [description] => [parent] => 119 [count] => 33 [filter] => raw ) [91] => WP_Term Object ( [term_id] => 78 [name] => Mobile Phase Suggestions [slug] => 58 [term_group] => 0 [term_taxonomy_id] => 78 [taxonomy] => category [description] => [parent] => 71 [count] => 15 [filter] => raw ) [92] => WP_Term Object ( [term_id] => 231 [name] => MTCP [slug] => 112 [term_group] => 0 [term_taxonomy_id] => 231 [taxonomy] => category [description] => [parent] => 210 [count] => 3 [filter] => raw ) [93] => WP_Term Object ( [term_id] => 233 [name] => Online Book of Standards [slug] => 148 [term_group] => 0 [term_taxonomy_id] => 233 [taxonomy] => category [description] => [parent] => 232 [count] => 2 [filter] => raw ) [94] => WP_Term Object ( [term_id] => 2 [name] => ONLINE TOOLS [slug] => 57 [term_group] => 0 [term_taxonomy_id] => 2 [taxonomy] => category [description] => [parent] => 0 [count] => 2 [filter] => raw ) [95] => WP_Term Object ( [term_id] => 232 [name] => Packaging Department [slug] => 111 [term_group] => 0 [term_taxonomy_id] => 232 [taxonomy] => category [description] => [parent] => 210 [count] => 3 [filter] => raw ) [96] => WP_Term Object ( [term_id] => 168 [name] => Partisil & Partisphere FAQ [slug] => 157 [term_group] => 0 [term_taxonomy_id] => 168 [taxonomy] => category [description] => [parent] => 253 [count] => 8 [filter] => raw ) [97] => WP_Term Object ( [term_id] => 144 [name] => Photochemical Reactor [slug] => 27 [term_group] => 0 [term_taxonomy_id] => 144 [taxonomy] => category [description] => [parent] => 139 [count] => 16 [filter] => raw ) [98] => WP_Term Object ( [term_id] => 72 [name] => Prep Chromatography [slug] => 75 [term_group] => 0 [term_taxonomy_id] => 72 [taxonomy] => category [description] => [parent] => 75 [count] => 4 [filter] => raw ) [99] => WP_Term Object ( [term_id] => 244 [name] => Pricing Policies [slug] => pricing-policies [term_group] => 0 [term_taxonomy_id] => 244 [taxonomy] => category [description] => [parent] => 224 [count] => 0 [filter] => raw ) [100] => WP_Term Object ( [term_id] => 210 [name] => Procedures Internal [slug] => 104 [term_group] => 0 [term_taxonomy_id] => 210 [taxonomy] => category [description] => [parent] => 5 [count] => 4 [filter] => raw ) [102] => WP_Term Object ( [term_id] => 234 [name] => Purchasing Department [slug] => 107 [term_group] => 0 [term_taxonomy_id] => 234 [taxonomy] => category [description] => [parent] => 210 [count] => 8 [filter] => raw ) [103] => WP_Term Object ( [term_id] => 171 [name] => Qualification FAQ [slug] => 16 [term_group] => 0 [term_taxonomy_id] => 171 [taxonomy] => category [description] => [parent] => 119 [count] => 60 [filter] => raw ) [104] => WP_Term Object ( [term_id] => 73 [name] => Qualification Information [slug] => 74 [term_group] => 0 [term_taxonomy_id] => 73 [taxonomy] => category [description] => [parent] => 71 [count] => 18 [filter] => raw ) [105] => WP_Term Object ( [term_id] => 242 [name] => Qx2 [slug] => 178 [term_group] => 0 [term_taxonomy_id] => 242 [taxonomy] => category [description] => [parent] => 7 [count] => 1 [filter] => raw ) [106] => WP_Term Object ( [term_id] => 235 [name] => Receiving Department [slug] => 110 [term_group] => 0 [term_taxonomy_id] => 235 [taxonomy] => category [description] => [parent] => 210 [count] => 4 [filter] => raw ) [107] => WP_Term Object ( [term_id] => 253 [name] => Reversed Phase Columns FAQ [slug] => reversed-phase-columns-faq [term_group] => 0 [term_taxonomy_id] => 253 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [108] => WP_Term Object ( [term_id] => 125 [name] => RSA Vials FAQ [slug] => 78 [term_group] => 0 [term_taxonomy_id] => 125 [taxonomy] => category [description] => [parent] => 120 [count] => 20 [filter] => raw ) [109] => WP_Term Object ( [term_id] => 225 [name] => SAGE 100 [slug] => 121 [term_group] => 0 [term_taxonomy_id] => 225 [taxonomy] => category [description] => [parent] => 210 [count] => 2 [filter] => raw ) [111] => WP_Term Object ( [term_id] => 236 [name] => Sales Department Inbound [slug] => 105 [term_group] => 0 [term_taxonomy_id] => 236 [taxonomy] => category [description] => [parent] => 210 [count] => 24 [filter] => raw ) [112] => WP_Term Object ( [term_id] => 237 [name] => Sales Department Outbound [slug] => 120 [term_group] => 0 [term_taxonomy_id] => 237 [taxonomy] => category [description] => [parent] => 210 [count] => 1 [filter] => raw ) [113] => WP_Term Object ( [term_id] => 226 [name] => Sales Order Module [slug] => 125 [term_group] => 0 [term_taxonomy_id] => 226 [taxonomy] => category [description] => [parent] => 225 [count] => 3 [filter] => raw ) [114] => WP_Term Object ( [term_id] => 3 [name] => SALES POLICIES [slug] => 15 [term_group] => 0 [term_taxonomy_id] => 3 [taxonomy] => category [description] => [parent] => 0 [count] => 10 [filter] => raw ) [115] => WP_Term Object ( [term_id] => 238 [name] => SFDC Salesforce.com [slug] => 106 [term_group] => 0 [term_taxonomy_id] => 238 [taxonomy] => category [description] => [parent] => 210 [count] => 7 [filter] => raw ) [116] => WP_Term Object ( [term_id] => 239 [name] => Shipping Department [slug] => 108 [term_group] => 0 [term_taxonomy_id] => 239 [taxonomy] => category [description] => [parent] => 210 [count] => 17 [filter] => raw ) [117] => WP_Term Object ( [term_id] => 163 [name] => Silica-C FAQ [slug] => 39 [term_group] => 0 [term_taxonomy_id] => 163 [taxonomy] => category [description] => [parent] => 153 [count] => 10 [filter] => raw ) [118] => WP_Term Object ( [term_id] => 150 [name] => Sparging Stones FAQ [slug] => 144 [term_group] => 0 [term_taxonomy_id] => 150 [taxonomy] => category [description] => [parent] => 147 [count] => 16 [filter] => raw ) [119] => WP_Term Object ( [term_id] => 118 [name] => Syringe Filters and Filtration [slug] => 73 [term_group] => 0 [term_taxonomy_id] => 118 [taxonomy] => category [description] => [parent] => 4 [count] => 15 [filter] => raw ) [120] => WP_Term Object ( [term_id] => 149 [name] => Syringe Filters FAQ [slug] => 38 [term_group] => 0 [term_taxonomy_id] => 149 [taxonomy] => category [description] => [parent] => 147 [count] => 32 [filter] => raw ) [121] => WP_Term Object ( [term_id] => 142 [name] => Syringe Pump Handheld [slug] => 45 [term_group] => 0 [term_taxonomy_id] => 142 [taxonomy] => category [description] => [parent] => 7 [count] => 0 [filter] => raw ) [122] => WP_Term Object ( [term_id] => 209 [name] => Technical Support Dept Internal [slug] => 113 [term_group] => 0 [term_taxonomy_id] => 209 [taxonomy] => category [description] => [parent] => 5 [count] => 20 [filter] => raw ) [123] => WP_Term Object ( [term_id] => 228 [name] => Templates Internal [slug] => 122 [term_group] => 0 [term_taxonomy_id] => 228 [taxonomy] => category [description] => [parent] => 5 [count] => 15 [filter] => raw ) [124] => WP_Term Object ( [term_id] => 8 [name] => TG-14 In-line Degasser [slug] => 171 [term_group] => 0 [term_taxonomy_id] => 8 [taxonomy] => category [description] => [parent] => 7 [count] => 0 [filter] => raw ) [125] => WP_Term Object ( [term_id] => 141 [name] => Tips Liquid Handling [slug] => 48 [term_group] => 0 [term_taxonomy_id] => 141 [taxonomy] => category [description] => [parent] => 139 [count] => 5 [filter] => raw ) [126] => WP_Term Object ( [term_id] => 146 [name] => Tools [slug] => 88 [term_group] => 0 [term_taxonomy_id] => 146 [taxonomy] => category [description] => [parent] => 139 [count] => 7 [filter] => raw ) [127] => WP_Term Object ( [term_id] => 217 [name] => Tools & Equipment Internal [slug] => 130 [term_group] => 0 [term_taxonomy_id] => 217 [taxonomy] => category [description] => [parent] => 5 [count] => 3 [filter] => raw ) [128] => WP_Term Object ( [term_id] => 74 [name] => Trouble Shooting Suggestions [slug] => 50 [term_group] => 0 [term_taxonomy_id] => 74 [taxonomy] => category [description] => [parent] => 71 [count] => 68 [filter] => raw ) [129] => WP_Term Object ( [term_id] => 81 [name] => Tubing Information [slug] => 77 [term_group] => 0 [term_taxonomy_id] => 81 [taxonomy] => category [description] => [parent] => 4 [count] => 26 [filter] => raw ) [130] => WP_Term Object ( [term_id] => 75 [name] => TYPE-C Column Information [slug] => 53 [term_group] => 0 [term_taxonomy_id] => 75 [taxonomy] => category [description] => [parent] => 71 [count] => 44 [filter] => raw ) [131] => WP_Term Object ( [term_id] => 164 [name] => TYPE-C in General FAQ [slug] => 40 [term_group] => 0 [term_taxonomy_id] => 164 [taxonomy] => category [description] => [parent] => 153 [count] => 49 [filter] => raw ) [132] => WP_Term Object ( [term_id] => 126 [name] => U-2D Sample MGT FAQ [slug] => 172 [term_group] => 0 [term_taxonomy_id] => 126 [taxonomy] => category [description] => [parent] => 120 [count] => 11 [filter] => raw ) [133] => WP_Term Object ( [term_id] => 162 [name] => UDC-Cholesterol FAQ [slug] => 24 [term_group] => 0 [term_taxonomy_id] => 162 [taxonomy] => category [description] => [parent] => 153 [count] => 2 [filter] => raw ) [135] => WP_Term Object ( [term_id] => 260 [name] => Vial Centrifuge [slug] => vial-centrifuge [term_group] => 0 [term_taxonomy_id] => 260 [taxonomy] => category [description] => [parent] => 5 [count] => 1 [filter] => raw ) [136] => WP_Term Object ( [term_id] => 121 [name] => Vials & Caps General FAQ [slug] => 10 [term_group] => 0 [term_taxonomy_id] => 121 [taxonomy] => category [description] => [parent] => 120 [count] => 62 [filter] => raw ) [137] => WP_Term Object ( [term_id] => 218 [name] => VOIXIS Phone Internal [slug] => 129 [term_group] => 0 [term_taxonomy_id] => 218 [taxonomy] => category [description] => [parent] => 5 [count] => 16 [filter] => raw ) [138] => WP_Term Object ( [term_id] => 241 [name] => Warehousing [slug] => 159 [term_group] => 0 [term_taxonomy_id] => 241 [taxonomy] => category [description] => [parent] => 210 [count] => 1 [filter] => raw ) [139] => WP_Term Object ( [term_id] => 240 [name] => Websites [slug] => 115 [term_group] => 0 [term_taxonomy_id] => 240 [taxonomy] => category [description] => [parent] => 210 [count] => 1 [filter] => raw ) [140] => WP_Term Object ( [term_id] => 83 [name] => White Papers [slug] => 52 [term_group] => 0 [term_taxonomy_id] => 83 [taxonomy] => category [description] => [parent] => 4 [count] => 6 [filter] => raw ) [141] => WP_Term Object ( [term_id] => 137 [name] => Window Maker FAQ [slug] => 41 [term_group] => 0 [term_taxonomy_id] => 137 [taxonomy] => category [description] => [parent] => 128 [count] => 21 [filter] => raw ) [142] => WP_Term Object ( [term_id] => 227 [name] => Work Order [slug] => 136 [term_group] => 0 [term_taxonomy_id] => 227 [taxonomy] => category [description] => [parent] => 225 [count] => 10 [filter] => raw ) [143] => WP_Term Object ( [term_id] => 138 [name] => Zero Flow Capillary FAQ [slug] => 42 [term_group] => 0 [term_taxonomy_id] => 138 [taxonomy] => category [description] => [parent] => 128 [count] => 20 [filter] => raw ) ) POST QUERY: POST QUERY RESULTS
ARTICLES, APPNOTES, FAQ
INTERNAL ONLY – CONFIDENTIAL
ONLINE TOOLS
SALES POLICIES

April 14, 2020
Bill Ciccone

How To... / HPLC Columns FAQ / HPLC Tips and Suggestions

This is most likely due to “channeling” of the flow pattern inside the column.

The packed bed uniformity has likely been disrupted and the split peaks and high pressure are indicative of this. In this case, the column will need to be replaced.

Download this Page

If you can’t find what you are looking for, email us

Related Articles

1) Lower than Normal Back Pressure seen using Cogent Diamond Hydride HPLC Columns. – FAQ

2) Restoring a blocked frit in an HPLC column. – Tips

3) HPLC Stationary Phase can be Responsible for a Blocked Frit- Tips & Suggestions

Contact Us