What are your definitions of normal phase reversed phase and ANP in HPLC – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 14, 2020
Bill Ciccone

ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Suggestions and Information / HPLC Tips and Suggestions

We use the short, quick and easy definitions shown below when describing our columns and when offering tips and suggestions.

Normal Phase Chromatography
Using Normal Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer.

Reversed Phase Chromatography
Using Reverse Phase Solvents:
• Most retained compounds are least polar.
• Least retained compounds are most polar.
• More Polar Solvents (water) Make compounds retain longer.

Aqueous Normal Phase Chromatography ANP
Using Reversed Phase Solvents:
• Most retained compounds are most polar.
• Least retained compounds are less polar.
• More Non Polar Solvents make compounds retain longer.

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