The following graph shows a study investigating the day to day retention time Precision of two test solutes on the Cogent Diamond Hydride 2.o™ Column.
Many factors can affect column robustness and lifetime, so it is not possible to define specific values for those parameters.
Mobile Phase:
A: DI H2O + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
Flow Rate: 0.3 mL/min
Injection Volume: 0.2uL
Detection: UV 254nm
Gradient:
Time(min) %B
0 90
6 20
7 20
8 90