Cogent Diamond Hydride 2.o HPLC column retention time precision – AppNote

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ARTICLES, APPNOTES, FAQ (9012)
- AppNotes Library (1878)
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November 13, 2015
Bill Ciccone

2.o™ HPLC Columns FAQ / AppNotes Cogent Diamond Hydride / AppNotes Library / ARTICLES, APPNOTES, FAQ / TYPE-C Column Information

This graph is from a study investigating the day to day retention time precision with two test solutes on the Cogent Diamond Hydride 2.o™ HPLC column

Peaks:
Blue Trace: Phenylalanine
Red Trace: Phenylglycine

Many factors can affect column robustness and lifetime, so it is not possible to define specific values for those parameters.

Mobile Phase:
—A: DI Water / 0.1% Formic Acid
—B: Acetonitrile / 0.1% Formic Acid
Flow Rate: 0.3mL / minute
Injection Volume: 0.2uL
Detection: UV 254nm

Gradient:

Time (minutes)	%B
0	90
6	20
7	20
8	90

Cogent Diamond Hydride HPLC Column Ordering Information

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