Increased Efficiency: 4 μm to 2.2 μm

The two chromatograms compare the retention and efficiency of a Cyclobenzaprine peak using two types of Cogent Diamond Hydride columns. Figure A uses a near UHPLC 2.2µm phase while Figure B uses a standard 4µm particle size column. The results show consistent retention between the two phases (about 5% difference).

These method conditions can readily be transferred from the 4um column to the 2.2um; the advantage of the 2.o™ phase is the significantly higher efficiency.


Method Conditions
Column: Cogent Diamond Hydride™, 2.2μm, 120Å
Catalog No.: 70200-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
A: 90% DI Water / 10% Acetonitrile / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)

Time (Minutes) %B
0 90
0.5 90
8 40
9 40

Post Time: 3 minutes
Flow rate: 0.3mL / minute
Detection: UV @ 230 nm
Injection vol.: 1μL
Sample Preparation:
10mg strength Cyclobenzaprine tablet was ground and added to a 50 mL volumetric flask. A diluent of 80/20/0.1 acetonitrile / DI water / formic acid was added and the flask was sonicated for 30 minutes. It was then diluted to mark, mixed, and filtered with a 0.45 µm nylon syringe filter (MicroSolv Tech Corp)

Note: Cyclobenzaprine is used to relieve muscle spasms and accompanying acute pain caused by various musculoskeletal maladies. Brand names include Amrix®, Flexeril®, and Fexmid®.


Method Transfer Cyclobenzaprine   Download File