L-Theanine in Green Tea Analyzed with HPLC – Extended AppNote

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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November 3, 2014
Bill Ciccone

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Food and Beverages / AppNotes Library / ARTICLES, APPNOTES, FAQ

L-Theanine in Green Tea

L-Theanine does not have significant UV absorption, but detection at a low wavelength (200 nm) was found to be suitable. With an expected sample concentration of around 10ppm, a calibration curve in the range 2–200 ppm would be appropriate.

The data showed good linearity in the studied range, with a line of best fit of y = 12.32x – 7.643 and an r2 value of 0.99995.

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