Separation by Functional Groups
Two proprietary compounds, which are precursors for a catalyst or Prodrugs are analyzed using a C18 Column under Normal Phase conditions. Separation of the two compounds is extremely reproducible (%RSD 0.1) and is very easy. The amount of moisture in your Mobile Phase is not an issue with this Method.
Peaks:
1. Compound 1
2. Compound 2
Method Conditions
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase: 95% Hexane / 5% Ethyl Acetate
Injection vol.: 1µL
Flow rate: 1mL / minute
Detection: Mass Spectrometer – Atmospheric Pressure Chemical Ionization in positive mode: APCI+ Single Ion Monitoring
Sample Preparation: 1mg / mL of proprietary compound. 1 (m/z 324) and 2 (m/z 228) dissolved in the Mobile Phase.
Notes: Because silanols on the Silica surface are substituted with Si-H, Water is not retained by the Stationary Phase, so drying of all the Solvents is not essential and analyses are very reproducible.
Attachment
No 23 Substituted Phenols Analyzed with HPLC pdf 0.2 Mb Download File
