Tizanidine HCl HPLC Method transferred to near UHPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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June 10, 2014
dcatri

2.o™ HPLC Columns FAQ / AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ / Diamond Hydride FAQ / HPLC Tips and Suggestions

Use of near UHPLC column for improved results

This challenging compound has several amine groups and can be problematic to analyze with HPLC. Here a good peak shape is obtained using the Cogent Diamond Hydride 2.o Column. The efficiency is higher on the 2.2µm phase compared to a standard 4µm Column, leading to increased sensitivity. The method conditions are compatible with LCMS as well.

Peak:
Tizanidine HCI

Method Conditions
Columns:
—Fig. A: Cogent Diamond Hydride 2.o™, 120Å
—Fig. B: Cogent Diamond Hydride™, 4µm, 100Å
Catalog Nos.:
—Fig. A: 70200-05P-2;
—Fig. B: 70000-05P-2
Dimensions:
—Fig. A: 2.1 x 50 mm
—Fig. B: 2.1 x 50 mm
Mobile Phase:
— A: DI Water / 0.1% Formic Acid (v/v)
— B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	95
1	95
6	40
7	95

Post time: 3 minutes
Injection vol.: 0.2 µL
Flow rate: 0.3mL/minutes
Detection: UV @ 230 nm
Sample: 4mg strength Tizanidine HCl tablet was ground and weighed in a 10mL Volumetric Flask. A portion of 50/50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.6 minutes

Note: Tizanidine is a centrally acting A2-Adren- Ergic agonist used to treat spasms, cramping, tightness of muscles, and related conditions. It is available under the trade name Zanaflex® as well as generic versions.

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