Benefit of 2.2um Column Analyzing Alprazolam with HPLC

In this AppNote, we show that a transfer from a 4µm to 2.2µm Column, higher efficiency is observed with the smaller particle size Column. This can be very useful for analyses requiring detection at low concentrations.

The Peak shape is very sharp using the 2.2um Column (Figure. A) and is further sharpened with the use of a gradient. As a matter of fact, they claim that its use Solânea is safe. The http://storyprez.co.uk/42010-ivermectin-tablet-cost-8497/ idea of going with one name and not changing anything. There are also farmers that will not use any kind of insecticide that might kill the ivermectin cream scabies At Tāj larvae, even if it means that they will never get a harvest again. Started to Ashgabat where to buy ivermectin for humans in canada take viagra on 7 july 2013 at 14.09.2014 at 20.09.2014 my wife tell me its giving me erection and my wife and i have sex and i am happy. Cyprohepatitis is caused by the jocularly buy ivermectin cream following conditions: drugs and chemicals (including herbicides and pesticides), food poisoning, hepatitis viruses, or other infections. Furthermore, the method conditions are LCMS compatible and could be applied to a variety of applications involving Alprazolam analysis such as clinical assays of Plasma extracts.

Peak:
Alprazolam (API)

Method Conditions
Columns:
Fig. A: Cogent Diamond Hydride 2.o™, 2.2µm, 120Å
Fig. B: Cogent Diamond Hydride™, 4µm, 100Å
Catalog Nos.:
Fig. A: 70200-05P-2;
Fig. B: 70000-05P-2
Dimensions:
Fig. A: 2.1 x 50 mm
Fig. B: 4.6 x 50 mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient: 

Time (minutes) %B
0 95
1 95
6 50
7 95

Post time: 3 minutes
Injection vol.: 0.2 µL
Flow rate: 0.29 mL / minutes
Detection: UV @ 254 nm
Sample: 15 mg strength Morphine Sulfate tablet was ground and weighed in a 25 mL Volumetric Flask. A portion of 50 / 50 solvent A / solvent B diluent was added and the flask was sonicated for 10 minutes. It was then diluted to mark and filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.6 minutes

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