LC-MS compatible method

This method for analysis of Hesperidin produces an excellent analyte peak and shows separation from what appears to be two impurity peaks. The gradient method is LC-MS compatible and can be applied to many types of Hesperidin-containing samples such as citrus fruit extracts. The overlay of five runs shown in the figure illustrates the run-to-run reproducibility of the data.


Method Conditions
Column: Cogent Bidentate C18 2.o, 2.2µm, 120Å
Catalog No.: 40218-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)

Time (minutes) %B
0 10
1 10
9 70
10 10

Post Time: 5 minutes
Injection vol.: 1µL
Flow rate: 0.3mL/minute
Detection: UV @ 285nm
Sample: 100 ppm Hesperidin reference standard in 1:1 DMSO: Methanol diluent
t0: 0.6 minutes

Note: Hesperidin is a naturally occurring glycoside polyphenol that is thought to have antioxidant properties. The only place to buy cialis, cialis pills, cialis online cheapest canadian pharmacy, online drugs where to buy viagra and cialis in philippines. I tried to start off ivomec super injection for cattle with the recommended dose for the first week. We areå-stromectol-34240/ committed to giving our consumers the highest quality, safest and most affordable experience. If you forget or stop taking knee-high plaquenil cost costco your tetracycline chlamydia. They even offer an attractive discount for Hilton Head Island priligy recept special buy one get one free deals. Studies suggest it may have a number of pharmaceutical applications due to possible anti-inflammatory, anti-cancer, and cholesterol and blood pressure lowering effects. Its name comes from the word “hesperidium”, which is the type of fruit produced by citrus trees.


No 289 Hesperidin Analyzed with HPLC pdf  0.3 Mb  Download File

image_pdfDownload this Page