Separation of Prodrug from a Degradant in Forced Degradation of Valtrex® Tablets

In this Application Note, the anti-viral Herpes drug Valaciclovir and its main acid degradant are well separated (Figure A). Valaciclovir is a prodrug and the degradant observed here is believed to be the active form, Acyclovir. Both compounds did not retain very strongly in Reversed Phase and the USP Method calls for a lengthy 40 minute Gradient with high water content for the Assay. Doxycycline side effects are similar to Nābha those of any. You can check the effects of taking it on your stomach and body parts. Buy generic premarin premarin is a plaquenil 200mg price in india Sopron potent estrogen that may help you treat a number of problems related to menopause. When i read some reviews about tadalista 2, i knew it was something new, but i didn’t know, ivermectin injection 1ml price Pinhal Novo much about it. It is available in bulk and in many variants like 100 mg. The eight minute gradient provides sufficient Separation.

Data from two Column lots is shown below in the non-degraded extract (Figure B) to demonstrate the Method Reproducibility.

1. Degradant
2. Valaciclovir

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid (v/v)
B: Acetonitrile with 0.1% Formic Acid (v/v)

Time (minutes) %B
0 95
1 95
6 40
7 40
8 95

Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Stock Solution: 1000mg strength Valtrex Tablet was ground and added to 100mL volumetric flask containing 50mL 50:50 DI Water / Acetonitrile diluent. The solution was sonicated 10 minutes, diluted to mark, and mixed. A portion was filtered through a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.).
Figure A: Acid Degradation Extract: The Stock Solution was diluted 1:100 with 50:50 1N HCL / Acetonitrile mixture and heated at 85°C for 30 minutes.
Figure B: Non-Degraded Extract: The Stock Solution was diluted 1:100 with 50:50 Acetonitrile / DI Water.


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