Basic compound functional group dependent adsorption study in RSA autosampler vials – AppNote

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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November 26, 2013
Bill Ciccone

AppNotes Library / AppNotes RSA Vials / ARTICLES, APPNOTES, FAQ / Autosampler Vials FAQ / Autosampler Vials Information / Crimp Top Vials & Cap FAQ / Diamond Hydride FAQ / Frequently Asked Questions?? / How To... Information / HPLC Suggestions and Information / Inserts FAQ / MicroSolv Autosampler Vials FAQ / RSA Family of Vials / RSA Vials FAQ / U-2D Sample MGT FAQ / Vials & Caps General FAQ

Adsorption study comparing RSA™ to conventional glass autosampler vials.

Various test solutes were investigated in this study in order to determine the role that compound specific functional groups play in analyte loss to a vial. Percent loss increased in order of increasing number of amine froups and was greatest with permanently cationic species (thiamine and cetylpyridinium chloride).

Furthermore, percent loss was significantly lower using RSA™ glass vials, which show how the surface chemistry has real end user advantages for the analytical laboratory. This is due to the fact that RSA glass vials do not have the many surface hydroxyl groups (aKa silanols) that are found in both standard glass vials and market leading certified glass vials. RSA Study Table

Chemical_structures_used_in_study_graphic

Method Conditions
Tested Items : RSA™, Glass Vials and AQR™ Screw Caps v. Market Leading Vials.
Specifications: Reduced Surface Activity Glass, Clear, 2ml, Write-On, Screw Top Vials and AQR Caps
Catalog No.: 9509S-1WCP-RS
Columns:
–Diamond Hydride™, 4μm,100Å
–Bidentate C18™, 4μm, 100Å
Catalog Nos.:
—70000-10P
—40018-10P
Mobile Phase: Various Isocratic settings were used
Flow rate: 1.0mL / minute
Sample Preparation: 5.0ppm reference standards in DI water diluent. Portions of the same samples were transferred to the two vial types and injected into an HPLC initially and again after four hours. Peak areas were recorded and compared to initial injections to calculate percent recovery.

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