Suggestion for separation of phenylephrine HCl acetaminophen and dextromethorphan – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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January 11, 2013
Bill Ciccone

AppNotes Pharmaceuticals / Bidentate C18 FAQ / Cogent Bidentate C18 Information / Cogent Phenyl Hydride Information / HPLC Column Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Phenyl Hydride FAQ / Trouble Shooting Suggestions / TYPE-C Column Information

The Cogent Phenyl Hydride™ would be recommended for these compounds because of dextromethorphan.

It has a strong tendency to tail on most columns but the Phenyl Hydride™ column can produce a very symmetrical peak; a mobile phase additive of 0.1% TFA would be beneficial as well for reducing dextromethorphan peak tailing.

Phenylephrine HCL does not normally retain very well in Reversed Phase so a starting mobile phase of ~95% aqueous is suggested. The dextromethorphan is more hydrophobic and so a gradient going to ~50% organic would be recommended. Be sure not to start the gradient until after the phenylephrine elutes or you may get less retention. Acetaminophen shows moderate retention and good separation should be achievable between it and phenylephrine with a high water content mobile phase.

The Cogent Bidentate C18™ column is another alternative. It is more hydrophobic than the Cogent Phenyl Hydride™ so you may get better retention of the phenylephrine and acetaminophen. However, dextromethorphan will likely have more tailing than with the Cogent Phenyl Hydride™.

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