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Formulas for chromatography

Reference Number: AA-02820 Created: 06/24/2016 03:37 PM Last Updated: 07/23/2016 09:47 PM

Efficiency (Theoretical Plates):  
N = 5.54  (  t/ w1/22
5.54 is a constant when using the "Half Height" method. tR is the retention time of the peak and w1/2 is the width at half height.


Capacity Factor (k'):
  
k' =  (t— t0) / t0
t0 = (the volume of the mobile phase inside the column) / (Flow Rate)
t= Band Retention Time


Selectivity (Alpha Value):
  
a = k2 / k1  

Peak Asymmetry:  
As = B/A (at 10% peak height) 

B = At 10% peak height, the width of a line from the slope to a perpendicular line drawn from the peak's height to the baseline @ 10% height. B is the tailing end of the chromatogram.
A = At 10% peak height, the width of a line from the slope to a perpendicular line drawn from the peak's height to the baseline @ 10% height. A is the fronting part of the chromatogram.

 

Resolution:  
Rs = (1/4)(a-1)(N)1/2[k/(1+k)] 
k = the average of two peaks. 

   
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