Triton X-100 gradient assay with HPLC- AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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October 26, 2022
Tanya

AppNotes ANP/HILIC Methods / AppNotes Cogent Bidentate C8 / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals

A reproducible assay for detection of a non-ionic surfactant.

Triton X-100 is used in the sample purification steps of various materials as well as for cleaning apparatus in pharmaceutical manufacturing. Detection and quantification are very important to identify any residual amounts of this compound.

The overlay of 5 injections (below) demonstrates reproducibility in separating the polyoxyethylene mixture.

Peak:
Triton X-100

Method Conditions:
Column: Cogent Bidentate C8™, 4μm, 100Å
Catalog No.: 40008-10P
Dimensions: 4.6 x 100mm
Mobile Phase:
—A: DI water with 0.1% formic acid
—B: Acetonitrile with 0.1% formic acid

Time (minutes)	%B
0	98
2	95
3	95
5	95
10	98

Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 230nm
Sample Preparation: Triton X-100, 10uL / mL in DI water

Note: Triton X-100 is one of the most widely used nonionic surfactants for lysing cells to extract protein and other cellular organelles. Triton X-100 (C₁₄H₂₂O(C₂H₄O)ₙ) has a hydrophilic polyethylene oxide chain and an aromatic hydrocarbon lipophilic or hydrophobic group. The hydrocarbon group is a 4-phenyl group.

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