Ticagrelor Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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September 10, 2021
Tanya

AppNotes Cogent Phenyl Hydride / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

A Reproducible Method for Detection of this Blood Clot Preventative
Click HERE for Column Ordering Information.

A rapid, sensitive, and Reproducible Method has been developed for analysis of Ticagrelor. The data below, (overlay of 5 chromatograms ) illustrates how the compound can be adequately Retained and detected using a simple Gradient in Reversed Phase HPLC. The Method demonstrates good Peak Shape and run-to-run Precision with RSD values less than 0.3%.

A Phenyl ring in the Column Stationary Phase provides beneficial π-π Interaction with the Analyte making possible the use of a very simple, Mass Spec friendly Mobile Phase with Formic Acid as an additive.

5 Injections of Ticagrelor Overlay

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-10P-2
Dimensions: 2.1mm x 100mm
Mobile Phase:
—A: DI Water with 0.1% Formic Acid (v/v)
—B: Acetonitrile with 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	40
1	40
5	85
6	85
7	40
10	40

Injection vol.: 1μL
Flow rate: 0.2mL / minute
Detection: UV @ 254nm
Sample Preparation: 2.0mg / mL Ticagrelor standard solution in Methanol.

Notes: Ticagrelor is an oral prescription indicated for treatment of acute ischemic stroke, coronary artery disease and acute coronary syndrome.

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