Retention of Erythromycin analyzed with ELSD – AppNote

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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June 16, 2022
Tanya

AppNotes Cogent Diamond Hydride / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Erythromycin, a macrolide antibiotic

As this compound lacks chromophores, it typically requires derivatization for use in UV detection. In this method, we retain excellent peak shape without the need for these pre-column derivatization steps saving time and resources.

RSD values (less than 0.5%) demonstrate the consistent and reliable retention, as shown in the 10 injections overlay below.

Peak:
Erythromycin

Method Conditions:
Column: Cogent Diamond Hydride™, 4μm, 100Å.
Catalog No.: 70000-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 50% acetonitrile / 50% DI water / 0.1% formic acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute.
Detection: ELSD, gain: 9, temperature: 50°C, nitrogen: 3.5 bar.
Sample Preparation: 2.0mg / mL Erythromycin HCL in DI water.

Notes: Erythromycin is a macrolide antibiotic used to treat bacterial infections and is also used to prevent recurrent rheumatic fever.

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