A Straightforward HPLC-UV method for Guanidine

A simple Method was developed for the analysis of Guanidine, a highly basic (pKa 12.5), and polar compound. Pre-column derivatization was not used in this method, but if higher sensitivity is needed for lower concentrations, ELSD could be employed. 




1. Impurity 
2. Guanidine

Method Conditions
Column: Cogent Diamond Hydride™, 4µm, 100Å
Catalog No.: 70000-10P
Dimensions: 4.6 mm x 100mm
Mobile Phase: 50% DI Water / 50% Acetonitrile / 0.1% Formic Acid
Injection vol.: 1µL
Flow rate: 1.0 mL / minute
Detection: UV @195 nm
Sample Preparation: 1.7 mg/mL Guanidine in Mobile phase

Note: This method could be used for clearance testing of guanidine in biopharmaceutical products.