Guanidine analyzed with HPLC- AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9187)
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February 21, 2024
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AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Listed by Column / AppNotes Listed by Compound / AppNotes Listed by Discipline / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

A Straightforward HPLC-UV method for Guanidine

A simple Method was developed for the analysis of Guanidine, a highly basic (pKa 12.5), and polar compound. Pre-column derivatization was not used in this method, but if higher sensitivity is needed for lower concentrations, ELSD could be employed.

Peaks:
1. Impurity
2. Guanidine

Method Conditions
Column: Cogent Diamond Hydride™, 4µm, 100Å
Catalog No.: 70000-10P
Dimensions: 4.6 mm x 100mm
Mobile Phase: 50% DI Water / 50% Acetonitrile / 0.1% Formic Acid
Injection vol.: 1µL
Flow rate: 1.0 mL / minute
Detection: UV @195 nm
Sample Preparation: 1.7 mg/mL Guanidine in Mobile phase

Note: This method could be used for clearance testing of guanidine in biopharmaceutical products.

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