Fluphenazine HCl USP Tablet Assay with HPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 24, 2021
Tanya

AppNotes Alternate Generic Methods / AppNotes Cogent RP C8 / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Alternate L7 Column used for the USP Assay Method of Fluphenazine HCl Tablets

This USP Fluphenazine HCl Tablet Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.2%. This demonstrates a good alternate Column for your Fluphenazine HCl Tablet USP Method.

Peak:

Fluphenazine HCl

Method Conditions:
Column: Cogent RP C8™, 5um, 150Å
Catalog No.: 68508-15P
Dimensions: 4.6mm x 150mm
Mobile Phase: 0.2% Triethylamine (TEA) in Diluent
—Buffer: 0.05 M Monobasic Potassium Phosphate adjusted with Phosphoric Acid to a pH of 2.5.
—Diluent: Acetonitrile, Methanol, and Buffer ( 30:30:40 )
Injection vol.: 25μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Fluphenazine Hydrochloride 60μg / mL in Diluent

Note 1: Fluphenazine Hydrochloride is the Hydrochloride salt of Fluphenazine, a Phenothiazine with Antipsychotic activity and potential Antineoplastic activity.

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