Fluorouracil Assay Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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August 17, 2021
Tanya

AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / AppNotes RP C18 / ARTICLES, APPNOTES, FAQ

A Reliable Method for a Chemotherapeutic Drug

This Fluorouracil Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.1%. This Method shows reliability for analysis of this Pyrimidine Analog that is an anti-neoplastic anti-metabolite.

Peak:

Fluorouracil

Method Conditions:
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-25P
Dimensions: 4.6mm x 250mm
Mobile Phase: (5:95) Methanol / Buffer
—Buffer: 0.73g / L of Monobasic Sodium Phosphate and 1.4g / L of Dibasic Sodium Phosphate in Water.
Injection vol.: 20μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Fluorouracil 1.0mg / mL in DI Water

Note: Fluorouracil is a Pyrimidine Analog used to treat Basal Cell Carcinomas, and as an injection in palliative Cancer treatment.

This Method was developed by and is presented courtesy of ARL- Eutech Scientific Services, Inc.

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