Reasons for using direct adaptive Di-Ad HPLC column connectors – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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February 28, 2022
Tanya

AppNotes Cogent Bidentate C8 / Chromatography Accessories & Supplies Information / Di-Ad Column Connector Information / Equipment FAQ / Fittings & Tubing for HPLC FAQ / Frequently Asked Questions?? / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Tubing Information

How to reduce peak broadening due to poor column connections

The spring-loaded “Direct Adaptive” Di-Ad™ HPLC column connectors were designed for speed and convenience when changing between different column brands on your instrument.

In the example below, we demonstrate how this error-free process using DiAd fittings can also reduce peak broadening from common poor connections.

Trace	Fitting Used	Height (mAU)	Plates (N)
Black	PEEK	31.33	6306
Red	Poorly Inserted PEEK	30.36	6023
Blue	Di-Ad™	35.37	7160

Method Conditions:
Direct Adaptive HPLC column connector: Double end fitting
Catalog No.: 49910-10-DD
Dimensions: 0.010″ ID x 1/16th” OD, 100mm long
Column: Cogent Bidentate C8™, 4μm, 100Å.
Catalog No.: 40008-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 40% acetonitrile / 60% DI water 0.1 % formic acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV 254nm
Sample Preparation: 0.1mg / mL phenol in mobile phase.

Notes: Column efficiency: theoretical plates (N) 1/2 Height N = 5.54((t_R/W)²)
t_R = Retention time of peak
W = Width of peak measured at 1/2 height

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