Adenosine USP Assay – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9410)
- AppNotes Library (1905)
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  - AppNotes Listed by Column (491)
    
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    AppNotes TYPE-C Columns (396)
    
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    AppNotes Cogent Bidentate C8 300 (5)
    
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- Autosampler Vials Information (323)
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  - HPLC & GC Standard Vials Caps & Inserts (66)
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- Frequently Asked Questions?? (3444)
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  - CE and CZE FAQ (176)
    
    Buffers for CE FAQ (18)
    
    Controlled Flow Capillary FAQ (32)
    
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  - Equipment FAQ (97)
    
    Knitted Reactor Coils (14)
    
    Tips Liquid Handling (7)
  - Filtration FAQ (175)
    
    Chrom Syringes FAQ (7)
    
    Cogent Column Filters – FAQ (24)
    
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  - HPLC Columns FAQ (819)
    
    2.o™ HPLC Columns FAQ (99)
    
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March 21, 2025
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AppNotes Alternate Generic Methods / AppNotes Library / AppNotes Listed by Column / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / AppNotes RP C18 / ARTICLES, APPNOTES, FAQ

Alternate L1 Column used for a USP Assay

This application note illustrates the USP method for Adenosine using the Cogent RP Similars C18 ODS column. The chromatograms demonstrate that the column meets the USP system suitability requirements, including tailing factor, resolution, and relative standard deviation. This makes it an excellent alternative for a USP Adenosine assay.

Peak 1.

Method Conditions:
Column: Cogent RP Hyper Similars C18™ 5um, 100A
Catalog No.: 62518-25P
Dimensions: 4.6 mm x 250 mm
Sample Preparation:— 0.2 mg/mL of Adenosine in Mobile phase
System suitability solution: 4 μg/mL each of Adenine and inosine in Mobile phase (peak order respectively.)

Resolution: ≥ 1.5 between adenine and inosine,
Tailing factor: ≤ 0.99
Relative standard deviation: 0.5%,

—

Note 1: For the full method conditions and details, consult the official United States Pharmacopeia–National Formulary (USP–NF.)

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