Percentage of acid needed in Aqueous Normal Phase ANP methods – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 14, 2020
Bill Ciccone

ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Cogent Diamond Hydride Information / Cogent Phenyl Hydride Information / Cogent Silica-C Information / Cogent UDA Information / Diamond Hydride FAQ / Frequently Asked Questions?? / HPLC Column Information / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Phenyl Hydride FAQ / Sales & Marketing Information / Trouble Shooting Suggestions / TYPE-C Column Information / TYPE-C in General FAQ

Generally speaking there is not much need to go above 0.5% acetic or formic acid for most ANP applications. If trifluoroacetic acid (TFA) is used such as in biological applications, the usual limit is about 0.1%. TFA is generally not recommended for LCMS because of the ion suppression it can cause.

Below are charts showing that low levels of formic acid are often better than higher levels. This is typical of ANP methods.

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