Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 14, 2020
Bill Ciccone

ANP Aqueous Normal Phase Articles / Frequently Asked Questions?? / HPLC Tips and Suggestions / TYPE-C Column Information

The highest polarity is generally found in ionized compounds due to the fixed charge on the compound.

Therefore in Aqueous Normal Phase ANP HPLC, strongest retention is attained with the highest polarity, when the compounds are fully ionized.

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