What is the difference between weak and strong cation exchange for Ion Exchange columns – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 14, 2020
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / General Information / HPLC Column Information / HPLC Columns FAQ / HPLC Primer / HPLC Tips and Suggestions / Prep Chromatography Column Information

An HPLC stationary phase is considered a weak cation exchanger when it has a functional group such as a carboxylic acid which proton does not come off (ionize) until around pH 4 or so.

Columns with sulfonic acid groups for example, are considered strong cation exchangers because they are essentially un-protonated (ionized) at any reasonable mobile phase pH for HPLC.

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