The pH range of common buffers for HPLC. – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ
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April 14, 2020
jimmy

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions

The following table shows a list of common buffers and their working buffer ranges:

Buffer	pH Range
Trifluoroacetic acid (TFA)	1.5-2.5
Phosphoric acid/monobasic phosphate (pKa 1)	1.1-3.1
Formic acid/formate	2.8-4.8
Acetic acid/acetate	3.8-5.8
Mono/dibasic phosphate (pKa 2)	6.2-8.2
Ammonia	8.2-10.2
1-methylpiperidine	9.1-11.1
Triethylamine	10.0- 12.0

As for concentration, about 5mM is suitable for Cogent TYPE-C Silica™ HPLC Columns while 25mM can be used with columns based on type B silica.

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