What are the pH ranges of common buffers for HPLC – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ
INTERNAL ONLY – CONFIDENTIAL
ONLINE TOOLS
SALES POLICIES

April 14, 2020
Bill Ciccone

ARTICLES, APPNOTES, FAQ / HPLC Column Information / HPLC Information and Suggestions / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Trouble Shooting Suggestions

The table below shows a list of common buffers and their working ranges for HPLC:

Buffer	pH Range
Trifluoroacetic acid TFA	1.5-2.5
Phosphoric acid/monobasic phosphate (pKa 1)	1.1-3.1
Formic acid/formate	2.8-4.8
Acetic acid/acetate	3.8-5.8
Mono/dibasic phosphate (pKa 2)	6.2-8.2
Ammonia	8.2-10.2
1-methylpiperidine	9.1-11.1
Triethylamine TEA	10.0- 12.0

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