How does the Cogent UDA HPLC column’s ligand bond to the silica hydride surface – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 14, 2020
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Cogent UDA Information / HPLC Column Information / HPLC Tips and Suggestions / TYPE-C Column Information / TYPE-C in General FAQ

The simple answer is directly to the silica surface by silicon to carbon bonds

For more details, the Cogent UDA™ HPLC column ligand is a C11 chain with a carboxylic acid on one end. On the other end, there are two orientations for how the ligand could be bonded to the Cogent TYPE-C™ Silica support.

The first orientation is shown in A. In this case, only one hydrosilation occurs from the starting reagent, resulting in a remaining double bond and a single point of attachment.

In the second orientation B, another hydrosilation occurs with structure A and a nearby Si-H group, resulting in no more double bonds. This requires that the other Si-H group is close enough to react. This is called a Bidentate attachment.

For the Cogent UDA™ HPLC columns, B is structure of the ligand.

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