How does ammonium fluoride affect HPLC and LCMS data if using Aqueous Normal Phase ANP methods – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 14, 2020
Bill Ciccone

ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Cogent Amide Information / Cogent Diamond Hydride Information / Cogent Phenyl Hydride Information / Cogent UDA Information / HPLC Column Information / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / TYPE-C Column Information

Ammonium fluoride is beneficial in several ways in an Aqueous Normal Phase ANP chromatographic method:

• As a buffer (i.e. pH control)
• Improvement of peak shape.
• Improvement of sensitivity in MS for certain compounds.

Using ammonium fluoride as a mobile phase additive is suggested for LCMS when using Aqueous Normal Phase HPLC methods. For further reading, please refer to the following third-party journal article reference:
J. Pesek, M. Matyska, “Ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography,” J. Chromatogr. A. 1401, 2015, 69–74.

NOTE: care should be taken when using ammonium fluoride because it is corrosive to glass (Teflon bottles are preferred) and using a concentration in excess of 1 mM may permanently damage the column. Cogent TYPE-C™ Silica columns last much longer than ordinary columns but ammonium fluoride may somewhat decrease the column lifetime.

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