Calculate the ligand density of a bonded phase of an HPLC column – How To

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 14, 2020
Bill Ciccone

2.o™ HPLC Columns FAQ / ARTICLES, APPNOTES, FAQ / Cogent UDC-Cholesterol Information / Frequently Asked Questions?? / How To... Information / HPLC Column Information / HPLC Columns FAQ / HPLC Primer / HPLC Tips and Suggestions / Reversed Phase Columns FAQ / TYPE-C Column Information / TYPE-C in General FAQ / UDC-Cholesterol FAQ

The ligand density can be calculated using the Berendsen-de Galan equation:

α = 10⁶ %C/(10² MW _carbon nC – %C MW _ligand) SBET

where α is the ligand density (µmol/m²), %C is the percent carbon (%), MW _carbon is the molecular weight of carbon (g/mol), nC is the number of carbon atoms per bonded ligand, MW _ligand is the molecular weight of the organic bonded ligand (g/mol), and SBET is the specific surface area of silica material (m²/g).

For example, the ligand density of the Cogent UDC-Cholesterol™ stationary phase comes out to approximately 1.5 µmol/m²using this equation.

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