Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 14, 2020
Bill Ciccone

2.o™ HPLC Columns FAQ / ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Cogent Diamond Hydride Information / Cogent Phenyl Hydride Information / Diamond Hydride FAQ / How To... Information / HPLC Column Information / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Phenyl Hydride FAQ / TYPE-C Column Information / TYPE-C in General FAQ

Many people ask me about the problem associated with polar acidic compound analysis using HILIC or ion exchange columns with Mass Spectrometry.

Fortunately, there is an easy solution to this problem. You can use Cogent TYPE-C HPLC columns such as the Diamond Hydride™, Phenyl Hydride™ and others to separate highly polar compounds in Aqueous Normal Phase ANP mode with better results. With ANP, you only need from 0.1mM to 10mM of ammonium acetate or other acids or bases to get great results.

This leads to many benefits such as minimizing MS inlet source build up of precipitated salt from using 20mM – 75mM as recommended by HILIC column suppliers. Also, too much salt in your mobile phase can cause ion suppression. This is avoided by adding the low amounts needed in ANP. Also, higher salt concentrations very often will corrode stainless steel frits, columns and system hardware if you don’t adequately flush the column and systems.

Below is an example of an MS Inlet that has been clogged by too much salt in a mobile phase.

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1) Why are Aqueous Normal Phase ANP HPLC methods better to use than HILIC – FAQ

2) Efficiency of ANP Measured – AppNote

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