Standard 4um Particle Size Transferred to 2.o™

This application illustrates how methods developed using the 4µm Cogent Diamond Hydride Columns may be adapted for Cogent Diamond Hydride 2.o™ phases. Morphine shows slightly higher retention on the 2.o™ Column (average 7.311 min vs. 5.588 min). The efficiency is almost twice as high when using the smaller particle sized column, demonstrating the excellent benefits of this option.

Three runs were performed on each column in order to demonstrate consistency.

Morphine sulfate

Method Conditions
Fig. A: Cogent Diamond Hydride 2.o™, 120Å
Fig. B: Cogent Diamond Hydride™, 4µm, 100Å
Catalog Nos.:
Fig. A: 70200-05P-2;
Fig. B: 70000-05P-3
Fig. A: 2.1 x 50 mm
Fig. B: 3.0 x 50 mm
Mobile Phase:
  A: DI Water / 0.1% Formic Acid (v/v)
—  B: Acetonitrile / 0.1% Formic Acid (v/v)

Time (minutes) %B
0 90
0.4 90
7.4 40
8.4 90

Post time: 5 minutes
Injection vol.: 0.2 µL
Flow rate:
Fig. A: 0.29mL / minutes
Fig. B: 0.50mL / minutes
Detection: UV @ 284nm
Sample: 15mg strength Morphine Sulfate tablet was ground and weighed in a 25mL volumetric flask. A portion of 50 / 50 Solvent A / Solvent B diluent was added and the flask was sonicated for 10 minutes. Solution was then diluted to mark and filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.4 minutes

Note: Morphine is a highly potent opiate analgesic widely used in clinical applications to treat severe pain. However, tolerance and addiction develop rapidly with its use so it has potential for abuse as well. It is named after Morpheus, the Greek god of dreams.


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