Prasugrel Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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October 25, 2016
dcatri

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

Comparison of Diamond Hydride to C18 HPLC Column

Prasugrel has a tertiary Amine and therefore tails in many Reversed Phase methods and conventional HPLC columns. Figure A shows how a sharp and symmetrical peak can be easily obtained with the Cogent Diamond Hydride Column using an MS-compatible Mobile Phase.

Figure B shows the peak that was obtained using a Reversed Phase gradient (30–60%B over 5 minutes) using the same Mobile Phase solvents and a Type B silica based C18 Column. Here the effects of residual silanols on the Type B silica based column lead to peak tailing.

Peak:
Prasugrel

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0.1% Formic Acid (v/v)
—B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (Minutes)	%B
0	97
1	97
5	60
6	97

Post Time: 2 minutes
Flow rate: 1.0 mL/minute
Detection: 254 nm
Injection vol.: 1μL
Sample Preparation: 0.1 mg/mL Prasugrel in Methanol diluent
t0: 0.9 minutes

Note: Prasugrel is a platelet inhibitor which was approved by the US Food and Drug Administration in 2009 for the reduction of thrombotic cardiovascular events. It is marketed as Effient®.

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